麻酔下全動物標本による「骨格筋DRG ニューロン」のホールセルパッチクランプ記録

• 八木 淳一

  杏林大学 医学部 統合生理学

• 小林 靖

  防衛医科大学校 解剖学講座

• 平井 直樹

  杏林大学 医学部 統合生理学

書誌事項

タイトル別名

• <b>Whole cell patch-clamp recordings of skeletal muscular </b><b>DRG neurons in anesthetized whole </b><b>animal preparations </b>

抄録

   Dorsal root ganglion (DRG) neurons that innervate the skeletal muscle (SK-DRG neurons) are differentiated into various subpopulations and each exhibits different receptive properties expressing different ion channels in different combinations. In this study, we introduce a novel method for in vivo patch-clamp recording of the SK-DRG neurons in rats to allow integrated analysis of their diverse properties.<br>   To identify the SK-DRG neurons, the fluorescent retrograde tracer DiI was injected into the left anterior tibial muscle of 6-7-week-old rats anesthetized with pentobarbital sodium. Three to 6 weeks after the injection, the rats were deeply anesthetized again, and each animal was mounted on the stage of an upright microscope. After isolating the left L4-DRG along with the sciatic nerve, maintaining the peripheral connection, it was placed in a chamber on the stage. The individual neurons on the ganglionic surface were then visualized.Whole-cell patch-clamp recording was established from the DiI-positive DRG neurons.<br>   Small- and medium-sized DRG neurons, which are mainly associated with nociception, could be classified into subpopulations based on axonal conduction velocity, receptive properties (mechanical and thermal stimuli), and current expression profiles (hyperpolarization-activated current and instantaneous current). Some of them responded to experimental ischemic muscle contraction. Therefore, we discuss some components that could cause muscle pain during ischemic contraction.<br>   Our novel method is useful for analyzing the diverse properties of SK-DRG neurons and for ascertaining the factors that cause pain in pathological conditions such as ischemia.

収録刊行物

• PAIN RESEARCH

  PAIN RESEARCH 24 (3), 109-116, 2009

  日本疼痛学会

キーワード

• In vivo patch clamp
• Primary afferent
• Muscle pain
• Ischemia
• Arteriosclerosis obliterans

詳細情報

• CRID: 1390001204366955136
• NII論文ID: 130004651900
• NII論文ID: 10025639080
• NII書誌ID: AN10195934
• DOI: 10.11154/pain.24.109
• ISSN: 21874697
• ISSN: 09158588
• Web Site: https://www.jstage.jst.go.jp/article/pain/24/3/24_1/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2009332763
• 本文言語コード: ja
• データソース種別:
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• 抄録ライセンスフラグ: 使用不可