Multiple reaction monitoring as a mean of validation tool for plasma biomarker discoverey with non-labeled peptides.

• Sasaki Koh-Ichi

  Department of Biomedical Engineering, Sapporo Medical University School of Medicine [in Japanese]

• Ozasa Yasuhiro

  [in Japanese]

• Kimura Michitoshi

  Department of Biomedical Engineering, Sapporo Medical University School of Medicine

• Sohma Hitoshi

  Department of Biomedical Engineering, Sapporo Medical University School of Medicine

• Aoshima Masato

  [in Japanese]

• Kokai Yasuo

  Department of Biomedical Engineering, Sapporo Medical University School of Medicine

Bibliographic Information

Other Title

• Ｍｕｌｔｉｐｌｅ　ｒｅａｃｔｉｏｎ　ｍｏｎｉｔｏｒｉｎｇをバリデーションに用いた質量分析による血漿バイオマーカー探索システムの検討
• Multiple reaction monitoring オ バリデーション ニ モチイタ シツリョウ ブンセキ ニ ヨル ケッショウ バイオマーカー タンサク システム ノ ケントウ

Abstract

Recent progress in mass spectrometry has made a major impact for discovery of plasma biomarkers. Although sensitivity of mass spectrometer for proteomics seemed to be very high, it still remained to be limited in quantification. This has hampered to validate molecular candidates in clinical samples. A validation system with high efficiency and throughput has been expected. </br>Multiple reaction monitoring (MRM) is a unique mode for quantitative analysis in mass spectrometry and being useful to quantify known analytes. We employed MRM as a mean of validation on possible marker peptides obtained by Q-TOF screening with non-labeled plasma proteins, and found that MRM validation in combination with Q-TOF screening might be an useful method to explore plasma biomarkers. Plasma proteins of mouse model for postmenopausal osteoporosis were separated by SDS-PAGE and in-gel digested to peptide with trypsin. These peptides were compared to those of control mice by ESI-Q-TOF. Mass spectra more than 3 fold increase in disease model were assigned by MS / MS analysis and used to construct MRM channels. The resulting MRM channels were directly used to validate their discriminatory power to distinguish disease conditions from control. </br>Construction of MRM channels with spectra from peptide assignment and direct use of MRM channels in biomarker validation appeared to be very effective. Collectively, MRM validation in combination with Q-TOF screening might be a suitable setting for a discovery of biomarker in any kind of body fluid including plasma.<br>

Journal

• SEIBUTSU BUTSURI KAGAKU

  SEIBUTSU BUTSURI KAGAKU 53 (1), 1-7, 2009

  Japanese Electrophoresis Society

Keywords

• validation
• MRM
• Q-TOF
• biomarker
• plasma

Details

• CRID: 1390001204201290496
• NII Article ID: 130004456491
• NII Article ID: 10025707947
• NII Book ID: AN00129729
• DOI: 10.2198/sbk.53.1
• COI: 1:CAS:528:DC%2BD1MXjsFKrtrc%3D
• ISSN: 13499785
• ISSN: 00319082
• NDL BIB ID: 10221268
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10221268
• Web Site: https://search.jamas.or.jp/link/ui/2009143090
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed