Caveolae-Independent Activation of Protein Kinase A in Rat Neonatal Myocytes

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Author(s)

Abstract

Cardiomyocytes express both β<sub>1</sub>- and β<sub>2</sub>-adrenergic receptors, and these receptors play a differential role in chronotropic and inotropic effects of the heart. Caveolae are known as an important regulator of G-protein-coupled receptor signaling. In the present report, we examined whether caveolae have a role in β-adrenergic receptor-stimulated cAMP production and protein kinase A activation in neonatal myocytes. Isoproterenol-stimulated cAMP production was mediated by β<sub>1</sub>- and β<sub>2</sub>-subtypes, which depends on the receptor number of each subtype. However, protein kinase A activation was exclusively mediated by the β<sub>1</sub>-subtype. Disruption of caveolae by methyl-β-cyclodextrin treatment did not affect the relative contribution of subtypes to isoproterenol-stimulated cAMP production. β<sub>1</sub>-Subtype-mediated protein kinase A activation was also not affected by the disruption of caveolae. These results suggest that β<sub>1</sub>-adrenergic receptor-mediated protein kinase A activation is compartmentalized and independent of caveolae.<br>

Journal

  • Journal of Pharmacological Sciences

    Journal of Pharmacological Sciences 98(2), 168-174, 2005-06-20

    The Japanese Pharmacological Society

References:  24

Codes

  • NII Article ID (NAID)
    10025728483
  • NII NACSIS-CAT ID (NCID)
    AA11806667
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    13478613
  • NDL Article ID
    7337896
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  NDL  J-STAGE 
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