Ephedorae herba Decreases Lipopolysaccharide-Induced Cyclooxgenase-2 Protein Expression and NF-.KAPPA.B-Dependent Transcription in C6 Rat Glioma Cells

• Aoki Kohichi

  Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University

• Yamakuni Tohru

  Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University

• Yoshida Masaaki

  Research Laboratory, Kotaro Pharmaceutical Co., Ltd.

• Ohizumi Yasushi

  Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University

Bibliographic Information

Other Title

• Ephedorae herba Decreases Lipopolysaccharide-Induced Cyclooxgenase-2 Protein Expression and NF-κB-Dependent Transcription in C6 Rat Glioma Cells
• Ephedorae herba Decreases Lipopolysaccharide Induced Cyclooxgenase 2 Protein Expression and NF カッパ B Dependent Transcription in C6 Rat Glioma Cells
• Ephedorae herba decreases lipoploysaccharide-induced cyclooxgenase-2 protein expression and NF-κB-dependent transcription in C6 rat glioma cells

Abstract

We studied the effects of Mao (Ephedrae herba) on lipopolysaccharide (LPS)-induced expression of inducible cyclooxygenase (COX-2) in C6 rat glioma cells. Western blot analysis showed that Mao inhibited LPS-induced expression of COX-2 protein, but not that of constitutive cyclooxygenase. Reporter gene assays showed that Mao reduced LPS-inducible NF-κB-dependent transcription that plays a crucial role in induction of COX-2 gene expression. This crude drug decreased LPS-induced IκBα degradation in a concentration-dependent manner without affecting LPS-induced IκB phosphorylation. These results suggest that Mao prevents LPS-induced NF-κB-dependent transcription by inhibiting IκB degradation and suppresses an increase in COX-2 protein expression in C6 cells.<br>

Journal

• Journal of Pharmacological Sciences

  Journal of Pharmacological Sciences 98 (3), 327-330, 2005

  The Japanese Pharmacological Society

Keywords

• <i>Ephedrae herba</i>
• COX-2 gene expression
• NF-κB-dependent transcription

Details

• CRID: 1390001205177018880
• NII Article ID: 10025729227
• NII Book ID: AA11806667
• DOI: 10.1254/jphs.sc0050118
• ISSN: 13478648
• ISSN: 13478613
• NDL BIB ID: 7369053
• PubMed: 16006736
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7369053
• Web Site: https://search.jamas.or.jp/link/ui/2006292587
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed