Involvement of Tryptase and Proteinase-Activated Receptor-2 in Spontaneous Itch-Associated Response in Mice With Atopy-like Dermatitis

• Tsujii Kenichiro

  Department of Applied Pharmacology, Graduate School of Medical and Pharmaceutical Science, University of Toyama, Japan

• Andoh Tsugunobu

  Department of Applied Pharmacology, Graduate School of Medical and Pharmaceutical Science, University of Toyama, Japan

• Ui Haruna

  Department of Applied Pharmacology, Graduate School of Medical and Pharmaceutical Science, University of Toyama, Japan

• Lee Jung-Bum

  Department of Pharmacognosy, Graduate School of Medical and Pharmaceutical Science, University of Toyama, Japan

• Kuraishi Yasushi

  Department of Applied Pharmacology, Graduate School of Medical and Pharmaceutical Science, University of Toyama, Japan

抄録

This study investigated the involvement of tryptase and proteinase-activated receptor (PAR) subtypes in spontaneous scratching, an itch-associated behavior, in NC mice. This strain of mice showed chronic atopy-like dermatitis and severe spontaneous scratching, when kept a long time in a conventional environment. The trypsin-like serine proteinase inhibitor nafamostat mesilate (1 – 10 mg/kg) dose-dependently inhibited spontaneous scratching in mice with dermatitis. The activity of tryptase was increased in the lesional skin, which was inhibited by nafamostat at a dose inhibiting spontaneous scratching. Enzyme histochemistry revealed the marked increase of toluidine blue–stained cells, probably mast cells, with tryptase activity in the dermis of the lesional skin. Intravenous injection of anti-PAR₂ antibody suppressed spontaneous scratching of mice with dermatitis. Intradermal injection of the PAR₂-activating peptide SLIGRL-NH₂, but not PAR₁, ₃, ₄-activating peptides, elicited scratching at doses of 10 – 100 nmol/site in healthy mice. PAR₂-immunoreactivity was observed in the epidermal keratinocytes in healthy and dermatitis mice. These results suggest that PAR₂ and serine proteinase(s), mainly tryptase, are involved in the itch of chronic dermatitis.<br>

収録刊行物

• Journal of Pharmacological Sciences

  Journal of Pharmacological Sciences 109 (3), 388-395, 2009

  公益社団法人 日本薬理学会

キーワード

• atopic dermatitis
• itch
• scratching
• tryptase
• proteinase-activated receptor

詳細情報

• CRID: 1390282680157676544
• NII論文ID: 10025735163
• NII書誌ID: AA11806667
• DOI: 10.1254/jphs.08332fp
• ISSN: 13478648
• ISSN: 13478613
• NDL書誌ID: 10188898
• Web Site: http://id.ndl.go.jp/bib/10188898
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10188898
• Web Site: https://search.jamas.or.jp/link/ui/2009231489
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可