Determination of platinum derived from cisplatin in human tissues using electrospray ionization mass spectrometry

• Minakata, Kayoko

• Nozawa, Hideki

• Okamoto, Naoko

• Suzuki, Osamu

Abstract

Determination of platinum (Pt) derived from cisplatin in tissues was performed by electrospray ionization mass spectrometry (ESI-MS) using silver (Ag) as the internal standard. Pt and Ag reacted with diethyldithiocarbamate (DDC), and were extracted using isoamylalcohol and acidified with oxalic acid. The compounds were termed Pt(DDC)3 + and Ag(DDC)2 +, based on their m/z values exhibiting the highest peaks at m/z 639 and m/z 405, respectively. The limit of detection was 30 pg and the quantitation range was from 100 to 10000 pg using 5 mg tissue. The present method allowed the determination of Pt in wet-ashed tissue in 10 minutes.

Journal

• Journal of Chromatography B

  Journal of Chromatography B 832 (2), 286-291, 2006-03-07

  Elsevier

Keywords

• Platinum
• Electrospray ionization
• Mass spectrometry
• Diethyldithiocarbamate
• Silver

Details

• CRID: 1050282676645704576
• NII Article ID: 10025780926
• NII Book ID: AA11656178
• ISSN: 1873376X
• ISSN: 15700232
• HANDLE: 10271/42
• Web Site: https://hama-med.repo.nii.ac.jp/records/28
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles
  • KAKEN