プロテオーム解析に基づく内耳特異的蛋白抗原の分離回収

• 富山 俊一

  日本医科大学多摩永山病院耳鼻咽喉科

書誌事項

タイトル別名

• Recovery of inner ear specific protein based on proteome profiling

抄録

In order to establish experimental inner ear specific autoimmune labyrinthitis in mice, the present study was designed to recover inner ear specific proteins from crude inner ear proteins by SDS-PAGE technique and proteome profiling. Thirty partitioned inner ear proteins according to molecular size were harvested through elution of crude inner ear antigens on Whole Gel Eluter. Serum from mice sensitized with 19 out of 30 partitions produced antibodies against bovine inner ear proteins by western blotting and 7 positive different bands were recognized. Proteome profiling of the several partitions that demonstrated clear positive bands were selectively carried out. Partition number 3, 10 and 11 hit only COCH protein. Partition number 5 and 8 hit COCH protein together with non specific inner ear proteins. Partition number 18, 22 and 24 hit non specific inner ear proteins. The present study succeeded in isolation of inner ear specific proteins, suggesting that development of inner ear specific autoimmune animal model is possible to elucidate pathogenesis of autoimmune inner ear disease.

収録刊行物

• Ｏｔｏｌｏｇｙ　Ｊａｐａｎ

  Ｏｔｏｌｏｇｙ　Ｊａｐａｎ 18 (2), 105-112, 2008

  一般社団法人　日本耳科学会

キーワード

• proteome profiling
• inner ear autoimmune disease
• animal model
• inner ear specific antibody
• COCH protein

詳細情報

• CRID: 1390001204769032192
• NII論文ID: 10025821389
• NII書誌ID: AN10358085
• DOI: 10.11289/otoljpn1991.18.2_105
• COI: 1:CAS:528:DC%2BD1cXnslKqtLc%3D
• ISSN: 18841457
• ISSN: 09172025
• Web Site: https://search.jamas.or.jp/link/ui/2008296333
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可