Age-Dependent Susceptibility to MPTP Neurotoxicity in C57BL Mice: A Tyrosine Hydroxylase-Immunohistochemical Evaluation
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- He Xi Jun
- Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- Nakayama Hiroyuki
- Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- Ueno Masaki
- Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- Doi Kunio
- Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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Abstract
The present study was designed to evaluate dopaminergic neuronal loss in the substantia nigra pars compact (SNpc) with immunohistochemical staining. C57BL/6 mice were intraperitoneally injected four times with 15 mg/kg 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), at 2 h intervals on 10 and 21 days, and 6, 12, 24 and 48 weeks of age. Animals were sacrificed 48 hours after the last injection. No change in the number of tyrosine hydroxylase (TH)-positive neurons was observed in 10- and 21-day-old mice after MPTP treatment compared with their corresponding controls. In contrast, MPTP produced a loss of 20.3% of TH-positive neurons in 6 week-old mice, and further decreases with advancing age, i.e., 35.8%, 39.9% and 56.2% TH-positive neuronal loss at 12, 24 and 48 weeks of age, respectively. These results provide evidence of age-related susceptibility of C57BL/6 mice to MPTP using TH immunohistochemistry. However, we failed to observe apoptosis of neurons in SNpc of mice of all ages after a subacute protocol of MPTP treatment (30 mg/kg/day × 5days).
Journal
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- Journal of Toxicologic Pathology
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Journal of Toxicologic Pathology 17 (4), 239-244, 2004
JAPANESE SOCIETY OF TOXICOLOGIC PATHOLOGY
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Details 詳細情報について
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- CRID
- 1390001204414292480
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- NII Article ID
- 10025852320
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- NII Book ID
- AA12022786
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- ISSN
- 1881915X
- 09149198
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed