Electron Microscopical Evidence of the Protective Function of Thioredoxin (TRX/ADF) Transgene against 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced Cellular Toxicity in the Liver and Brain

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Author(s)

    • YOON Byung-Il
    • Division of Cellular and Molecular Toxicology, Kangwon National University
    • KANEKO Toyozo
    • Division of Cellular and Molecular Toxicology, Kangwon National University
    • HIRABAYASHI Yoko
    • Division of Cellular and Molecular Toxicology, Kangwon National University
    • KODAMA Yukio
    • Division of Cellular and Molecular Toxicology, Kangwon National University
    • KANNO Jun
    • Division of Cellular and Molecular Toxicology, Kangwon National University
    • YODOI Junji
    • Department of Biological Responses, Institute for Virus Research, Kyoto University
    • HAN Jeong-Hee
    • Department of Veterinary Medicine, Kangwon National University
    • INOUE Tohru
    • Safety and Research Center of National Institute of Health Sciences

Abstract

The present study was performed to assess the protective role of thioredoxin/adult T-cell leukemia-derived factor (TRX/ADF) on the liver and brain cell damages induced by 2,3,7,8-tetrachlorodibenzo-<i>p</i>-dioxin (TCDD) in ADF wild-type (WT) and transgenic (Tg) mice. The ADF WT and Tg mice were intraperitoneally injected with a single dose of TCDD (150 μg/kg body weight). One day after the treatment, the liver and brain tissues were examined electron microscopically to evaluate the cellular toxicity. In the ADF WT mice, marked reduction of subcellular components, such as mitochondria, rough endoplasmic reticula, and glycogen granules, as well as swelling of the remaining mitochondria, were evident in the liver cells. However, attenuation of these changes was evident in TCDD-treated TRX/ADF mice. Similar subcellular changes noted in the neuronal cells of TCDD-treated WT mice were also attenuated in Tg mice. The results suggest that oxidative cellular damage contributes to the acute toxicity induced by TCDD and that TRX/ADF protects against it.

Journal

  • Journal of Toxicologic Pathology

    Journal of Toxicologic Pathology 18(1), 41-46, 2005-03-30

    JAPANESE SOCIETY OF TOXICOLOGIC PATHOLOGY

References:  33

Codes

  • NII Article ID (NAID)
    10025852625
  • NII NACSIS-CAT ID (NCID)
    AA12022786
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    0914-9198
  • Data Source
    CJP  J-STAGE 
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