The Foci of DNA Double Strand Break-recognition Proteins Localize with .GAMMA.H2AX after Heat Treatment

• TAKAHASHI Akihisa

  Department of Biology, School of Medicine, Nara Medical University

• MORI Eiichiro

  Department of Biology, School of Medicine, Nara Medical University

• OHNISHI Takeo

  Department of Biology, School of Medicine, Nara Medical University

Bibliographic Information

Other Title

• The foci of DNA double strand break-recognition proteins localize with γH2AX after heat treatment
• The foci of DNA double strand break recognition proteins localize with g H2AX after heat treatment

Abstract

Recently, there have been many reports concerning proteins which can recognize DNA double strand break (DSBs), and such proteins include histone H2AX phosphorylated at serine 139 (γH2AX), ataxia telangiectasia mutated (ATM) phospho-serine 1981, DNA-dependent protein kinase catalytic subunit (DNA-PKcs) phospho-threonine 2609, Nijmegen breakage syndrome 1 (NBS1) phospho-serine 343, checkpoint kinase 2 (CHK2), phospho-threonine 68, and structural maintenance of chromosomes 1 (SMC1) phospho-serine 966. Thus, it should be possible to follow the formation of DSBs and their repair using immunohistochemical methods with multiple antibodies to detect these proteins. When normal human fibroblasts (AG1522 cells) were exposed to 3 Gy of X-rays as a control, clearly discernable foci for these proteins were detected, and these foci localized with γH2AX foci. After heat treatment at 45.5°C for 20 min, these proteins are partially localized with γH2AX foci. Here we show that there were slight differences in the localization pattern among these proteins, such as a disappearance from the nucleus (phospho-ATM) and translocation to the cytoplasm (phospho-NBS1) at 30 min after heat treatment, and some foci (phospho-DNA-PKcs and phospho-CHK2) appeared at 8 h after heat treatment. These results are discussed from perspectives of heat-induced denaturation of proteins and formation of DSBs.

Journal

• Journal of Radiation Research

  Journal of Radiation Research 51 (1), 91-95, 2010

  Journal of Radiation Research Editorial Committee

Keywords

• Hyperthermia
• γH2AX
• Double strand break (DSB)
• DSB-recognition proteins

Details

• CRID: 1390282680193096064
• NII Article ID: 10025914249
• NII Book ID: AA00705792
• DOI: 10.1269/jrr.09111
• ISSN: 13499157
• ISSN: 04493060
• NDL BIB ID: 10540668
• PubMed: 20173316
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10540668
• Web Site: http://academic.oup.com/jrr/article-pdf/51/1/91/2732159/jrr-51-91.pdf
• Web Site: https://search.jamas.or.jp/link/ui/2010209555
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed