The Effects of X-Irradiation on Ex Vivo Expansion of Cryopreserved Human Hematopoietic Stem/Progenitor Cells

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Author(s)

Abstract

In our previous study (Life Sciences <b>84</b>: 598, 2009), we demonstrated that placental/umbilical cord blood-derived mesenchymal stem cell-like stromal cells have the effect to support the regeneration of freshly prepared X-irradiated hematopoietic stem/progenitor cells (HSPCs). Generally, HSPCs are supplied from companies, institutions, and cell banks that cryopreserve them for clinical and experimental use. In this study, the influence of cryopreservation on the responses of HSPCs to irradiation and co-culture with stromal cells is assessed. After cryopreservation with the optimal procedure, 2 Gy-irradiated HSPCs were cultured with or without stromal cells supplemented with combination of interleukin-3, stem cell factor, and thrombopoietin. The population of relatively immature CD34<sup>+</sup>/CD38<sup>–</sup> cells in cryopreserved cells was significantly higher than in fresh cells prior to cryopreservation; furthermore, the hematopoietic progenitor populations of CD34<sup>+</sup>/CD45RA<sup>+</sup> cells and CD34<sup>+</sup>/CD117<sup>+</sup> cells in cryopreserved cells were significantly lower than that in fresh cells. However, the rate of expansion in the cryopreserved HSPCs was lower than in the fresh HSPCs. In the culture of cryopreserved cells irradiated with 2 Gy, the growth rates of CD34<sup>+</sup> cells, CD34<sup>+</sup>/CD38<sup>–</sup> cells, and hematopoietic progenitors were greater than growth rates of their counterparts in the culture of fresh cells. Surprisingly, the effect to support the hematopoiesis in co-culture with stromal cells was never observed in the X-irradiated HSPCs after cryopreservation. The present results demonstrated that cryopreserving process increased the rate of immature and radio-resistant HSPCs but decreased the effects to support the hematopoiesis by stromal cells, thus suggesting that cryopreservation changes the character of HSPCs.

Journal

  • Journal of Radiation Research

    Journal of Radiation Research 51(2), 137-144, 2010-03-16

    Journal of Radiation Research Editorial Committee

References:  39

Codes

  • NII Article ID (NAID)
    10025914531
  • NII NACSIS-CAT ID (NCID)
    AA00705792
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    04493060
  • NDL Article ID
    10611704
  • NDL Source Classification
    ZS45(科学技術--医学--放射線医学)
  • NDL Call No.
    Z53-P232
  • DOI
    10.1269/jrr.09086
  • Data Source
    CJP  NDL  J-STAGE 
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