Effects of high-dose major components in oral disinfectants on the cell cycle and apoptosis in primary human gingival fibroblasts in vitro

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Author(s)

    • NOMURA Yuji
    • Department of Biomaterials Science, Graduate School of Biomedical Science, Hiroshima University
    • BHAWAL Ujjal Kumar
    • Department of Oral and Maxillofacial Surgery & High-Tech Research Center, Kanagawa Dental College
    • NISHIKIORI Ryo
    • Department of Biomaterials Science, Graduate School of Biomedical Science, Hiroshima University
    • SAWAJIRI Masahiko
    • Department of Oral and Maxillofacial Radiology, Graduate School of Biomedical Science, Hiroshima University
    • MAEDA Takeshi
    • Department of Prothetic Dentistry, Graduate School of Biomedical Science, Hiroshima University
    • OKAZAKI Masayuki
    • Department of Biomaterials Science, Graduate School of Biomedical Science, Hiroshima University

Abstract

We evaluated the effects of high-dose major components in oral disinfectants on oral cells from the standpoints of the cell cycle and apoptosis. We examined the viability and cell cycle of human gingival fibroblasts (HGFs) treated with the components of dental disinfectants, benzethonium chloride (BEC), benzalkonium chloride (BAC), and povidone iodine (PVD-I) using a cell counting kit and flow cytometry. The IC<Sub>50 </Sub>inhibitory concentration value in HGF cultures at 24 hours was 1.3×10<Sup>–2 </Sup>mM BEC, 6.0×10<Sup>–3 </Sup>mM BAC, and 2.6×10<Sup>–1 </Sup>mM PVD-I. In the cell cycle analysis, propidium iodide-stained HGFs were arrested in G<Sub>0</Sub>/G<Sub>1</Sub> of the cell cycle by all three disinfectants, and in the apoptosis assay, annexin V-FITC/PI-stained HGFs that became apoptotic at 5.0×10<Sup>–2 </Sup>and 1.0×10<Sup>–1 </Sup>mM BEC and 5.0×10<Sup>–2 </Sup>and 1.0×10<Sup>–1 </Sup>mM BAC, but not in PVD-I at concentrations as high as 5.0×10<Sup>–1 </Sup>mM. Our findings describe the effects of high-dose oral disinfectants, rather than clinical concentrations. Nevertheless, appreciating the effects of high-dose disinfectants absorbed into the human body is important, where they may accumulate in specific tissues and cells.

Journal

  • Dental Materials Journal

    Dental Materials Journal 29(1), 75-83, 2010-01-01

    The Japanese Society for Dental Materials and Devices

References:  36

Codes

  • NII Article ID (NAID)
    10025917650
  • NII NACSIS-CAT ID (NCID)
    AA10443149
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    02874547
  • Data Source
    CJP  J-STAGE 
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