Dry-Resistance of Salmonella enterica subsp. enterica Serovar Enteritidis is Regulated by Both SEp22, a Novel Pathogenicity-Related Factor of Salmonella, and Nutrients

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  • Tamura Ai
    Laboratory of Biodefense & Regulation, Osaka University of Pharmaceutical Sciences
  • Yamasaki Manabu
    Division of Biomedical Food Research, National Institute of Health Sciences
  • Okutani Akiko
    Division of Biomedical Food Research, National Institute of Health Sciences
  • Igimi Shizunobu
    Division of Biomedical Food Research, National Institute of Health Sciences
  • Saitoh Noriko
    Laboratory of Electron Microscopy, National Institute of Infectious Diseases
  • Ekawa Tomoya
    CAF Laboratories, Inc.
  • Ohta Hiroyuki
    CAF Laboratories, Inc.
  • Katayama Yoko
    Laboratory of Environmental Microbiology, Faculty of Agriculture, Tokyo University of Agriculture & Technology
  • Amano Fumio
    Laboratory of Biodefense & Regulation, Osaka University of Pharmaceutical Sciences

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Abstract

Environmental isolates of Salmonella enterica serover Enteritidis (S. Enteritidis) clones were grown to the logarithmic phase, washed and re-suspended in saline or Luria-Bertani (LB) medium, and then 10-μL aliquots of the suspensions were dried overnight at room temperature. The dried bacteria were mixed with 1 mL of ice-cold PBS, suspended and examined for colony-forming activity. All of the pathogenic clones with high levels of SEp22, identical to Salmonella Dps, maintained good viability if suspended in LB medium prior to drying. However, none of the non-virulent strains, exhibiting low levels of SEp22, survived. Similar results were obtained with sep22-knocked out mutants, suggesting that SEp22 is important for the acquisition of dry-resistance. Nutritional factors, such as LB medium, cabbage extracts, and egg yolk but not egg white, were shown to be necessary for the acquisition of dry-resistance, because none of the clones remained viable irrespective of SEp22 expression if suspended in saline. Scanning electron micrograms also supported the importance of nutrition, showing re-growth of the bacteria after drying in LB but not in saline. These results suggest the importance of both SEp22 expression and nutrients for the acquisition of dry-resistance by S. Enteritidis. <br>

Journal

  • Microbes and Environments

    Microbes and Environments 24 (2), 121-127, 2009

    Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

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