Analysis of Ammonia Monooxygenase and Archaeal 16S rRNA Gene Fragments in Nitrifying Acid-Sulfate Soil Microcosms
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- Nakaya Asami
- Graduate School of Bioresource Science, Nihon University
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- Onodera Yuki
- Graduate School of Bioresource Science, Nihon University
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- Nakagawa Tatsunori
- College of Bioresource Sciences, Nihon University
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- Satoh Kazuo
- Graduate School of Bioresource Science, Nihon University
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- Takahashi Reiji
- College of Bioresource Sciences, Nihon University
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- Sasaki Satohiko
- Advanced Research Institute, Nihon University
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- Tokuyama Tatsuaki
- College of Bioresource Sciences, Nihon University
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Abstract
The present study describes the occurrence of a unique archaeal ammonia monooxygenase alpha subunit (amoA) gene in nitrifying acid-sulfate soil microcosms at pH 3.5. The soil was collected from an abandoned paddy field in Thailand. Microcosms were incubated in the dark at 30°C for 372 days with the following three treatments: addition of ammonium sulfate solution once a month (I) or once a week (II), and addition of only sterilized water (III). A quantitative PCR analysis revealed an increase in abundance of the archaeal amoA gene in microcosm soils in which nitrate concentrations increased after incubation. A phylogenetic analysis indicated a predominance of the novel gene, and a predominance of a betaproteobacterial amoA gene affiliated with the genus Nitrosospira. A 16S rRNA gene-based PCR assay revealed that crenarchaeotic Group I.1d was predominant among the Crenarchaeota in microcosms. These results suggest the presence of ammonia-oxidizing archaea corresponding to the unique amoA lineage in nitrifying acid-sulfate soil microcosms at pH 3.5.<br>
Journal
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- Microbes and Environments
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Microbes and Environments 24 (2), 168-174, 2009
Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles
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Details 詳細情報について
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- CRID
- 1390282679322943872
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- NII Article ID
- 10025920328
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- NII Book ID
- AA11551577
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- ISSN
- 13474405
- 13426311
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- NDL BIB ID
- 10237050
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed