Analysis of Nanoplankton Community Structure Using Flow Sorting and Molecular Techniques

• Yoshida Noriaki

  Ocean Research Institute, University of Tokyo

• Nishimura Masahiko

  Ocean Research Institute, University of Tokyo

• Inoue Katsuyuki

  Ocean Research Institute, University of Tokyo

• Yoshizawa Susumu

  Ocean Research Institute, University of Tokyo

• Kamiya Eriko

  Ocean Research Institute, University of Tokyo

• Taniguchi Akito

  Laboratory of Environmental Science for Aquaculture Graduate School of Agriculture, Kinki University,

• Hamasaki Koji

  Ocean Research Institute, University of Tokyo

• Kogure Kazuhiro

  Ocean Research Institute, University of Tokyo

抄録

We developed a method for the separate and simultaneous analysis of the community structure of heterotrophic nanopkankton (HNP) and autotrophic nanoplankton (ANP). This method consists of three steps. First, nanoplankton cells were concentrated using a cross-flow filtration system because cell densities in natural seawater are usually too low for genetic studies. Second, HNP and ANP were separated by flow cytometric sorting ("flow sorting") on the basis of the presence or absence of chlorophyll. Finally, the community structure was analyzed using denaturing gradient gel electrophoresis targeting 18S rRNA gene. The newly developed method was applied to the coastal surface water of Aburatsubo Inlet, Japan, in July 2008. The separation of nanoplankton into HNP and ANP was validated by phylogenetic analysis, and the trophic mode of uncultured nanoplankton was confirmed (e.g. Marine Alveolata group II [MALV II] and Marine Stramenopile clade-2 [MAST-2]). This new method involving cell concentration, flow sorting and phylogenetic analysis is a potentially powerful tool for evaluating the population dynamics and ecology of marine protozoa.<br>

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 24 (4), 297-304, 2009

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• heterotrophic nanoplankton
• autotrophic nanoplankton
• cross-flow filtration
• flow sorting
• community structure

詳細情報

• CRID: 1390001204347211776
• NII論文ID: 10025921150
• NII論文ID: 130000133327
• NII書誌ID: AA11551577
• DOI: 10.1264/jsme2.me09150
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 10463063
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10463063
• Web Site: http://www.jstage.jst.go.jp/article/jsme2/24/4/24_ME09150/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可