Production and characterization of Brassica napus-Raphanus sativus monosomic addition lines mediated by the synthetic amphidiploid "Raphanobrassica"

• Akaba Michiko

  Laboratory of Plant Breeding, Faculty of Agriculture, Utsunomiya University United Graduate School of Agriculture, Tokyo University of Agriculture and Technology

• Kaneko Yukio

  Laboratory of Plant Breeding, Faculty of Agriculture, Utsunomiya University

• Ito Youichi

  Laboratory of Plant Breeding, Faculty of Agriculture, Utsunomiya University

• Nakata Yoshimi

  Laboratory of Plant Breeding, Faculty of Agriculture, Utsunomiya University

• Bang Sang Woo

  Laboratory of Plant Breeding, Faculty of Agriculture, Utsunomiya University

• Matsuzawa Yasuo

  Laboratory of Plant Breeding, Faculty of Agriculture, Utsunomiya University

抄録

In order to breed Brassica napus–Raphanus sativus monosomic addition lines (MALs), hybridizations between two synthetic amphidiploid (RRAA and RRCC) and B. napus (AACC) were performed. Two allooctoploids (RRAAAACC and AACCRRCC) were produced from each F₁ hybrid by chromosome doubling. From successive backcrosses to B. napus, MAL plants were first obtained in BC₂ generation and the R. sativus-derived chromosome was identified by genomic in situ hybridization (GISH). The nine chromosomes of R-genome in the MAL plants were clearly classified by each chromosome-specific RAPD markers. As a result, alloplasmic (radish cytoplasm) B. napus–R. sativus MAL having 8 types (a–i, except for h-type) and autoplasmic (rape cytoplasm) MAL with complete 9 types (a–i) were obtained in BC₃ and BC₄ generations. These alloplasmic and autoplasmic MAL plants were showed differences in their morphological, physiological and cytogenetical characters. From the survey of favorable traits, it was suggested that the a-type had fertility restoring gene(s) for male sterility in alloplasmic line and the g-type had a gene controlling white color petal. These two MALs are useful materials for exploring agronomic traits located on each chromosome of radish and for promoting the introgression of promising radish gene(s) to B. napus.<br>

収録刊行物

• Ｂｒｅｅｄｉｎｇ　Ｓｃｉｅｎｃｅ

  Ｂｒｅｅｄｉｎｇ　Ｓｃｉｅｎｃｅ 59 (2), 109-118, 2009

  日本育種学会

キーワード

• <i>Raphanus sativus</i>
• <i>Brassica napus</i>
• <i>Raphanobrassica</i>
• monosomic addition lines (MALs)
• random amplified polymorphic DNA (RAPD)
• genomic <i>in situ</i> hybridization (GISH)

詳細情報

• CRID: 1390282679699332480
• NII論文ID: 130004057038
• NII論文ID: 10025922735
• NII書誌ID: AA11317194
• DOI: 10.1270/jsbbs.59.109
• ISSN: 13473735
• ISSN: 13447610
• NDL書誌ID: 10239786
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10239786
• Web Site: http://www.jstage.jst.go.jp/article/jsbbs/59/2/59_2_109/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可