Effect of Varying HAP/TCP Ratios in Tape-cast Biphasic Calcium Phosphate Ceramics on Response In Vitro
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- Tanimoto Yasuhiro
- Department of Dental Biomaterials, Nihon University School of Dentistry at Matsudo
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- Shibata Yo
- Department of Oral Biomaterials and Technology, Showa University School of Dentistry
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- Murakami Asuka
- Department of Oral Biomaterials and Technology, Showa University School of Dentistry
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- Miyazaki Takashi
- Department of Oral Biomaterials and Technology, Showa University School of Dentistry
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- Nishiyama Norihiro
- Department of Dental Biomaterials, Nihon University School of Dentistry at Matsudo
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Abstract
The in-vitro response of the biphasic calcium phosphate (BCP) ceramics hydroxyapatite (HAP) and β-tricalcium phosphate (TCP) prepared by tape-casting was investigated. BCP ceramic slurries were prepared to HAP/β-TCP weight ratios of 75/25 (H75-T25), 50/50 (H50-T50), and 25/75 (H25-T75). Additionally, 100% HAP (H100) and 100% β-TCP (T100) were prepared as control. BCP ceramic sheets fabricated by tape-casting were sintered at 1200°C for 4 h. After sintering at 1200°C, thin-film X-ray diffraction confirmed that β-TCP in the BCP ceramics was partially transformed to α-TCP; HAP showed no change. Field-emission scanning electron microscope observation showed that the surfaces of H25-T75 and T100 were completely covered with precipitate after 90 d' immersion in phosphate-buffered saline solution with pH 7.4 at 37°C. After osteoblast-like (MC3T3-E1) cell cultivation for 7 d, H25-T75 showed a significantly higher cell number than H100, H75-T25, and H50-T50 (Fisher's PLSD test, p<0.05). These results indicate that the determination of an optimal balance of biological stability and biodegradation rate is important to fabricating BCP ceramics because it directly affects the in-vitro response. Within the limitation of this study, it is concluded that BCP ceramics with a HAP/TCP ratio of 25/75 prepared by a tape-casting technique has high cellular activity.
Journal
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- Journal of Hard Tissue Biology
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Journal of Hard Tissue Biology 18 (2), 71-76, 2009
THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY
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Details 詳細情報について
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- CRID
- 1390282679433866880
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- NII Article ID
- 10025927756
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- NII Book ID
- AA11074332
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- ISSN
- 1880828X
- 13417649
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- NDL BIB ID
- 10472811
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed