Superiority of Alkali-Extracted Rice Protein in Bioavailability to Starch Degraded Rice Protein Comes from Digestion of Prolamin in Growing Rats
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- KUMAGAI Takehisa
- Kameda Seika Co., Ltd.
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- WATANABE Reiko
- Course of Food and Nutrition, Department of Human Life Environments, Niigata Women's College
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- SAITO Mariko
- Kameda Seika Co., Ltd.
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- WATANABE Toshiyuki
- Kameda Seika Co., Ltd.
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- KUBOTA Masatoshi
- Laboratory of Nutritional Regulation, Faculty of Agriculture, Niigata University
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- KADOWAKI Motoni
- Laboratory of Nutritional Regulation, Faculty of Agriculture, Niigata University
Bibliographic Information
- Other Title
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- Superiority of alkali-extracted rice protein in bioavailability to starch degraded rice protein comes from the digestion of prolamin in growing rats
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Abstract
The rice protein prepared from alkaline extraction (AE-RP) has high digestibility compared to that obtained from starch degradation (SD-RP) in in vitro digestion experiments, and alterations in the protein body (PB) structures were observed in AE-RP in the previous study. The improvement in the digestibility of AE-RP is probably a result of the structural change of PB. The present study was carried out to elucidate the superiority of AE-RP compared to SD-RP in bioavailability in growing rats. There were no major differences between AE-RP and SD-RP in polypeptide compositions according to SDS-PAGE and their amino acid compositions. The equivalent body weight gain and similar growth curves in both AE-RP and casein (control) groups were obtained during the feeding period of 28 d, and their values were significantly higher compared to the SD-RP group (p<0.05). The protein efficiency ratio (PER) of the SD-RP (1.73) group was significantly lower than those of the AE-RP (1.87) and casein (1.84) groups (p<0.05). The plasma lysine concentrations at the last stage of the feeding period in the AE-RP and SD-RP groups were approximate levels and were appreciably lower, compared to that of the casein group (p<0.001). Portal plasma amino acid concentrations were determined after single administration (4 g/kg) of two rice proteins in non-anaesthetized rats. All the amino acid concentrations in the 2 groups reached a maximum level at 30 min or 1 h and decreased to the pre-administration levels 6 h after the start of administration. The total amounts of three amino acids, leucine, valine and arginine, which appeared in the portal blood during the 6 h period after the start of administration of AE-RP, were higher than those of SD-RP (p<0.05). Furthermore, 13 kDa prolamin was detected with Western-blot analysis only in the feces of rats fed SD-RP. Consequently, these results indicate that the bioavailability of rice protein containing prolamin was improved by alkaline extraction.
Journal
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- Journal of Nutritional Science and Vitaminology
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Journal of Nutritional Science and Vitaminology 55 (2), 170-177, 2009
Center for Academic Publications Japan
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Details 詳細情報について
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- CRID
- 1390001206324797056
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- NII Article ID
- 10025970960
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- NII Book ID
- AA00703822
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- COI
- 1:CAS:528:DC%2BD1MXlsVWhsbk%3D
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- ISSN
- 18817742
- 03014800
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- NDL BIB ID
- 10207881
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed