カップ積層型カーボンナノチューブの発光現象とマイクロチップ電気泳動への応用  [in Japanese] Luminescence of Cup-Stacked Carbon Nanotubes and Its Application to Microchip Electrophoresis  [in Japanese]

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Author(s)

    • 井上 陽介 INOUE Yousuke
    • 名古屋大学大学院工学研究科化学・生物工学専攻 Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
    • 岡本 行広 OKAMOTO Yukihiro
    • 名古屋大学大学院工学研究科化学・生物工学専攻 Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
    • 加地 範匡 [他] KAJI Noritada
    • 名古屋大学大学院工学研究科化学・生物工学専攻 Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
    • 渡慶次 学 TOKESHI Manabu
    • 名古屋大学大学院工学研究科化学・生物工学専攻 Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
    • 馬場 嘉信 BABA Yoshinobu
    • 名古屋大学大学院工学研究科化学・生物工学専攻 Department of Applied Chemistry, Graduate School of Engineering, Nagoya University

Abstract

従来,マイクロチップ電気泳動でレーザー励起蛍光検出を用いて高感度検出を試みる際に試料の蛍光標識を行う.しかし,蛍光標識は煩雑な操作,標識に伴う分離能の低下及び低濃度試料の蛍光誘導体化効率の低下という問題を有しており,蛍光標識を不要とする高感度検出法の開発が望まれている.著者らは遠心操作によりカップ積層型カーボンナノチューブ(cup stacked carbon nanotubes,CSCNTs)が蛍光を発することを見いだし,泳動液へのCSCNTsの添加によりDNAのマイクロチップ電気泳動分離及び非標識(間接蛍光)検出に成功した.CSCNTsは蛍光を有することに加えて,製造段階でのアスペクト比の調整やCSCNTs表面への機能性分子の固定化が容易であるためにマイクロチップ電気泳動のみならず様々な分野における活用が今後期待される.

Microchip electrophoresis (MCE) is one of the most suitable methods for biomolecular separation and analysis because of its superior characteristics. In biomolecular separation and analysis, laser induced fluorescent (LIF) detection is frequently employed in MCE and attains high sensitive detection. However, LIF detection requires the labeling of analytes, which contains troublesome procedures and results in decreasing of the separation efficiency. In addition, the labeling reaction at low concentration is significantly difficult, and thus true high sensitive detection is not easily attained and has been desired. Here, we report on the possibility of cup-stacked carbon nanotubes (CSCNTs) for high sensitive label-free detection and high separation efficiency. At first we investigated fluorescence properties of CSCNTs. Our investigation revealed that supernatant solution of CSCNTs after centrifugation had fluorescence at around 500 nm, while CSCNTs suspension did not. The application of CSCNTs for MCE enabled us to successfully separate and detect DNA without labeling it. Compared to the direct detection method, our method resulted in poor separation efficiency. However, by controlling of the CSCNTs' aspect ratio and the immobilization of functional molecules on the CSCNTs surfaces, we could improve separation efficiency and attain the separation of various samples without labeling samples.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 58(6), 517-521, 2009-06-05

    The Japan Society for Analytical Chemistry

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Codes

  • NII Article ID (NAID)
    10025979400
  • NII NACSIS-CAT ID (NCID)
    AN00222633
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    05251931
  • NDL Article ID
    10336476
  • NDL Source Classification
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL Call No.
    Z17-9
  • Data Source
    CJP  NDL  IR  J-STAGE 
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