Visual Determination of Trace Zinc(II) by Reverse Phased TLC with Tetrakis(4-carboxyphenyl)porphine

• Fujita Takako

  Graduate School of Science and Engineering, Yamagata University

• Murotsuki Sachie

  Graduate School of Science and Engineering, Yamagata University

• Tsuchiya Saori

  Graduate School of Science and Engineering, Yamagata University

• Mizuguchi Hitoshi

  Graduate School of Science and Engineering, Yamagata University

• Katagiri Hiroshi

  Graduate School of Science and Engineering, Yamagata University

• Shida Junichi

  Graduate School of Science and Engineering, Yamagata University

Bibliographic Information

Other Title

• テトラキス（４－カルボキシフェニル）ポルフィンを用いる逆相薄層クロマトグラフィーによる亜鉛イオンの目視定量
• テトラキス 4 カルボキシフェニル ポルフィン オ モチイル ギャクソウハクソウ クロマトグラフィー ニ ヨル アエン イオン ノ モクシ テイリョウ

Abstract

A new visual colorimetric and fluorimetric determination of trace zinc(II) has been developed by thin-layer chromatography (TLC) with tetrakis(4-carboxyphenyl)porphine (TCPP). In this method, the spots that are dependent on each metal ion concentration of zinc(II), copper(II), cadmium(II), and mercury(II) appear selectively on an octadecylsilanized (ODS) silica thin layer by chromatographic separation with 90% (w/w) methanol. The yellow-colored zinc(II)-TCPP complex is distinguished visually from the other species with different colors. The zinc(II) concentration is determined by a visual comparison of the yellow-colored spot. The visual detection limit is 3 × 10⁻⁶ mol dm⁻³, defined as the minimum concentration that is visually distinguishable against a reagent blank. The calibration curve assessed with the densitometric responses is linear in the concentration range 0∼5 × 10⁻⁶ mol dm⁻³. The detection limit, which is calculated with 3 σ at the reagent blank, is 3 × 10⁻⁷ mol dm⁻³. On the other hand, the fluorimetric detection of the zinc(II)-TCPP complex is also available under the irradiation of UV light in the dark, and the visual detection limits is 8 × 10⁻⁷ mol dm⁻³. The calibration curve in the fluorimetric mode is linear in the concentration range 0∼3 × 10⁻⁶ mol dm⁻³, and the limit of detection is 3 × 10⁻⁷ mol dm⁻³. The effect for the determination of zinc(II) is not found in the presence of magnesium(II), aluminum(III), calcium(II), chromium(III), iron(III), nickel(II), and cadmium(II). The proposed method has been successfully applied to the determination of zinc(II) in river-water samples around an old mine.

Journal

• BUNSEKI KAGAKU

  BUNSEKI KAGAKU 58 (8), 761-765, 2009

  The Japan Society for Analytical Chemistry

Keywords

• zinc ion
• TCPP
• thin-layer chromatography
• visual detection
• ODS silica plate

Details

• CRID: 1390001204355444736
• NII Article ID: 10025980126
• NII Book ID: AN00222633
• DOI: 10.2116/bunsekikagaku.58.761
• COI: 1:CAS:528:DC%2BD1MXhtVKltrjP
• NDL BIB ID: 10338404
• ISSN: 05251931
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10338404
• Web Site: http://www.jstage.jst.go.jp/article/bunsekikagaku/58/8/58_8_761/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed