「内耳形態と細胞機能の研究手法」７．パッチクランプ法

• 伊藤 健

  東京大学医学部附属病院耳鼻咽喉科

書誌事項

タイトル別名

• Patch Clamp Technique

抄録

The patch clamp technique enables the analyses of various ion channel characteristics of individual cells in vitro. The most important difference from previous techniques is that the microelectrode (pipette) is firmly attached to the cell surface instead of being inserted into the cells, and the so-called "giga-ohm seal" is attained. Usually, the whole conductance of the cell membrane is measured under "whole cell" and "perforated patch" modes, whereas single channel recordings are also possible by employing relevant modes such as the "cell attached" mode. In the most frequently used "whole cell" mode, the intracellular potential (voltage clamp) or the whole cell current (current clamp) can be kept constant, since the leakage current is very small. Exemplary recordings are also shown from the author's experiments.

収録刊行物

• Ｅｑｕｉｌｉｂｒｉｕｍ　Ｒｅｓｅａｒｃｈ

  Ｅｑｕｉｌｉｂｒｉｕｍ　Ｒｅｓｅａｒｃｈ 67 (4), 263-268, 2008

  一般社団法人 日本めまい平衡医学会

キーワード

• patch clamp
• giga-ohm
• whole cell mode
• perforated patch mode
• voltage clamp
• current clamp
• single channel recording

詳細情報

• CRID: 1390282679927640704
• NII論文ID: 10026087620
• NII書誌ID: AN00001485
• DOI: 10.3757/jser.67.263
• ISSN: 1882577X
• ISSN: 03855716
• Web Site: https://search.jamas.or.jp/link/ui/2008320685
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可