Infrared Super-Resolution Imaging of a Single A549 Cell by Fluorescence Detection

• Ohmori Tsutomu

  Department of Medical Engineering, National Defense Medical College

• Inoue Keiichi

  Chemical Resources Laboratory, Tokyo Institute of Technology

• Sakai Makoto

  Chemical Resources Laboratory, Tokyo Institute of Technology

• Fujii Masaaki

  Chemical Resources Laboratory, Tokyo Institute of Technology

• Ishihara Miya

  Department of Medical Engineering, National Defense Medical College

• Kikuchi Makoto

  Department of Medical Engineering, National Defense Medical College

Bibliographic Information

Other Title

• 蛍光検出を利用した赤外超解像顕微鏡法によるA549細胞の測定

Abstract

2-color infrared (IR) super-resolution microscopy enables us to detect IR absorption with resolution of less than 1 μm, which is equivalent to that of fluorescence microscope. To prove that this microscopy can be utilized as cell analysis, we attempted to detect IR absorption inside A549 cells at IR wavelength of about 3 μm. When stained, we achieved to detect the IR absorption of protein molecules bound to fluorescent molecules. When non-stained, we achieved to detect the IR absorption of flavin molecules using auto-fluorescence. The microscopy is applicable to biochemical analysis inside cells based on molecular structure and thereby e.g. to cell selection.

Journal

• Nippon Laser Igakkaishi

  Nippon Laser Igakkaishi 30 (4), 427-434, 2009

  Japan Society for Laser Surgery and Medicine

Keywords

• infrared microscopy
• super-resolution
• infrared absorption spectrum
• fluorescence staining
• auto-fluorescence
• A549 cells

Details

• CRID: 1390282679743679872
• NII Article ID: 10026089140
• NII Book ID: AN0035795X
• DOI: 10.2530/jslsm.30.427
• ISSN: 18811639
• ISSN: 02886200
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed