Virulence Change of Infectious Hematopoietic Necrosis Virus against Rainbow Trout Oncorhynchus mykiss with Viral Molecular Evolution

• Mochizuki Mamiko

  Fuji Trout Hatchery, Shizuoka Prefectural Research Institute of Fishery

• Kim Hyoung Jun

  Faculty of Fisheries Sciences, Hokkaido University

• Kasai Hisae

  Faculty of Fisheries Sciences, Hokkaido University

• Nishizawa Toyohiko

  Faculty of Fisheries Sciences, Hokkaido University

• Yoshimizu Mamoru

  Faculty of Fisheries Sciences, Hokkaido University

Bibliographic Information

Other Title

• 分子進化に伴い変化するIHNVの病原性

Abstract

Infectious hematopoietic necrosis virus (IHNV) is the causative agent of IHN, one of the most serious viral diseases of salmonid fish. A total of five major genogroups including JRt for Asian isolates were confirmed among worldwide isolates based on glycoprotein (G) gene nucleotide (nt) sequences. The present study revealed existence of new two lineages, JRt Shizuoka and JRt Nagano, in the genogroup JRt by addition of new isolates obtained in 2006. The maximum nt diversity of G gene within JRt Shizuoka or JRt Nagano lineage was 6.3% or 3.5%, while that between JRt Shizuoka and JRt Nagano lineages was 7.0%. To evaluate influence of the evolutional divergence to virulence of IHNV, experimental challenges to rainbow trout Oncorhynchus mykiss were conducted by bath exposure at 10⁴ TCID₅₀/mL of RtShiz06s and RtShiz06a (JRt Shizuoka lineage), RtNag96 and RtNag06a (JRt Nagano lineage), and ChAb76 (a representative of the genogroup U). Distinct difference was observed in IHNV virulence to rainbow trout, i.e. the highest virulence was in RtShiz06s and RtShiz06a (≥ 76% of mortalities), and subsequently in RtNag96 and RtNag06a (20-40%), but scarcely any virulence in ChAb76 (≤ 10%). Thus it was suggested that nt diversity of Japanese IHNV continued rapidly with changing its virulence in rainbow trout farm environments.

Journal

• Fish Pathology

  Fish Pathology 44 (4), 159-165, 2009

  The Japanese Society of Fish Pathology

Keywords

• infectious hematopoietic necrosis virus
• IHNV
• virulence
• rainbow trout
• phylogeny
• nucleotide diversity

Details

• CRID: 1390001206461792128
• NII Article ID: 10026105793
• NII Book ID: AN00063165
• DOI: 10.3147/jsfp.44.159
• ISSN: 18817335
• ISSN: 0388788X
• HANDLE: 2115/53174
• NDL BIB ID: 10528168
• Web Site: https://agriknowledge.affrc.go.jp/RN/2030782678
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10528168
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed