Effects of heme oxygenase-1 on tissue factor and thrombomodulin in the endothelial cell

• MARUYAMA Keiko

  Department of Laboratory Science, School of Health Sciences, Faculty of Medicine, Kanazawa University

• MORISITA Eriko

  Department of Laboratory Science, School of Health Sciences, Faculty of Medicine, Kanazawa University Department of Cellular Transplantation Biology, Kanazawa University Graduate School of Medical Science

• SEKIYA Akiko

  Department of Laboratory Science, School of Health Sciences, Faculty of Medicine, Kanazawa University

• NAGAYA Satomi

  Department of Laboratory Science, School of Health Sciences, Faculty of Medicine, Kanazawa University

• KADONO Tadaaki

  Department of Laboratory Science, School of Health Sciences, Faculty of Medicine, Kanazawa University

• ASAKURA Hidesaku

  Department of Cellular Transplantation Biology, Kanazawa University Graduate School of Medical Science

• NAKAO Shinji

  Department of Cellular Transplantation Biology, Kanazawa University Graduate School of Medical Science

• OHTAKE Sigeki

  Department of Laboratory Science, School of Health Sciences, Faculty of Medicine, Kanazawa University

• YACHIE Akihiro

  Angiogenesis and Vascular Development (Department of Pediatrics) Kanazawa University Graduate School of Medical Science

Bibliographic Information

Other Title

• ヘムオキシゲナーゼ－１（ＨＯ－１）による血管内皮細胞上の組織因子，　トロンボモジュリンの調節

Abstract

Heme oxygenases (HO) are rate-limiting enzymes that catalyze the conversion of heme into biliverdin, free iron and carbon monoxide. They exist in three different isoforms, HO-1, HO-2 and HO-3. Recently, Yachie et al. reported the first human case of HO-1 deficiency. In this child, both intravascular hemolysis and endothelial cell injury were prominent. In addition, abnormalities of coagulation/fibrinolysis system were marked. The aim of this study was to elucidate the effects of HO-1 or HO-1 products on expression of tissue factor (TF) and thrombomodulin (TM) in human endothelial cells (HUVEC), in vitro. HUVEC were incubated with either hemin (HO-1 inducer) or hemin/Tin protoporphyrin IX (SnPP, HO-1 inhibitor) at 37 °C for each hours. The levels of mRNA of HO-1, TF and TM were determined by real-time reverse transcriptase polymerase chain reaction. The levels of antigen of cell lysates were determined by ELISA. Hemin increased HO-1 and TM expression. On the other hand, hemin/SnPP increased TF expression and decreased TM expression. In conclusion, HO-1 or HO-1 products might regulate TF and TM expression and prevent thrombus formation in human endothelial cells.

Journal

• Japanese Journal of Thrombosis and Hemostasis

  Japanese Journal of Thrombosis and Hemostasis 20 (3), 315-322, 2009

  The Japanese Society on Thrombosis and Hemostasis

Keywords

• heme oxygenases-1
• human umbilical vein endothelial cells
• tissue factor
• thrombomodulin

Details

• CRID: 1390282679428379264
• NII Article ID: 10026117537
• NII Book ID: AN10353762
• DOI: 10.2491/jjsth.20.315
• ISSN: 18808808
• ISSN: 09157441
• Web Site: https://search.jamas.or.jp/link/ui/2009284155
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed