Triacylated lipopeptide, a component of Gram-positive bacteria, induces osteoclastogenesis in the absence of RANKL and resorbs calvarial bone in vivo through Toll-like receptor 2

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Author(s)

    • SUZUKI KEIKO
    • 昭和大学歯学部歯科薬理学 Department of Pharmacology, School of Dentistry, Showa University
    • YAMADA SHOJI
    • 昭和大学歯学部歯科薬理学 Department of Pharmacology, School of Dentistry, Showa University

Abstract

To study the involvement of lipopeptide, a component of Gram-positive bacteria, in the pathogenesis of inflammatory osteolysis including periodontitis and osteomyelitis, we investigated whether lipopeptide influenced the differentiation and activation of osteoclasts. Pam<sub>3</sub>CSK<sub>4</sub>, a synthetic triacylated lipopeptide which has been proved to activate macrophages through TLR2, stimulated osteoclastogenesis at all the concentrations examined (between 10 and 1000 ng/ml), without adding RANKL exogenously, in bone marrow-derived preosteoclasts. Moreover, local injection of Pam<sub>3</sub>CSK<sub>4</sub> to wild-type mouse induced osteolysis in calvaria, which was not observed in TLR2-null mouse, indicating that bone resorption <i>in vivo</i> also occurred through a TLR2-dependent mechanism. These findings demonstrate an important role of triacylated lipopeptide in differentiation and activation of osteoclasts which results in pathological bone destruction through TLR2 ligation.

Journal

  • ORAL THERAPEUTICS AND PHARMACOLOGY

    ORAL THERAPEUTICS AND PHARMACOLOGY 29(1), 9-14, 2010-04-01

    JAPANESE SOCIETY OF ORAL THERAPEUTICS AND PHARMACOLOGY

References:  17

Codes

  • NII Article ID (NAID)
    10026132647
  • NII NACSIS-CAT ID (NCID)
    AN0023517X
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    02881012
  • Data Source
    CJP  J-STAGE 
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