Triacylated lipopeptide, a component of Gram-positive bacteria, induces osteoclastogenesis in the absence of RANKL and resorbs calvarial bone <i>in vivo</i> through Toll-like receptor 2

• SUZUKI KEIKO

  昭和大学歯学部歯科薬理学

• YAMADA SHOJI

  昭和大学歯学部歯科薬理学

抄録

To study the involvement of lipopeptide, a component of Gram-positive bacteria, in the pathogenesis of inflammatory osteolysis including periodontitis and osteomyelitis, we investigated whether lipopeptide influenced the differentiation and activation of osteoclasts. Pam₃CSK₄, a synthetic triacylated lipopeptide which has been proved to activate macrophages through TLR2, stimulated osteoclastogenesis at all the concentrations examined (between 10 and 1000 ng/ml), without adding RANKL exogenously, in bone marrow-derived preosteoclasts. Moreover, local injection of Pam₃CSK₄ to wild-type mouse induced osteolysis in calvaria, which was not observed in TLR2-null mouse, indicating that bone resorption in vivo also occurred through a TLR2-dependent mechanism. These findings demonstrate an important role of triacylated lipopeptide in differentiation and activation of osteoclasts which results in pathological bone destruction through TLR2 ligation.

収録刊行物

• 歯科薬物療法

  歯科薬物療法 29 (1), 9-14, 2010

  日本歯科薬物療法学会

キーワード

• Toll-like receptor 2
• Pam₃CSK₄
• osteoclastogenesis
• RANKL-independent
• Gram-positive bacteria
• periodontitis

詳細情報

• CRID: 1390001204734590720
• NII論文ID: 10026132647
• NII書誌ID: AN0023517X
• DOI: 10.11263/jsotp.29.9
• COI: 1:CAS:528:DC%2BC3cXovFOjt7k%3D
• ISSN: 18844928
• ISSN: 02881012
• Web Site: https://search.jamas.or.jp/link/ui/2010293240
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可