Transgenic note: Modification of the surface carbohydrate composition of tobacco protoplasts transformed with the human UDP-galactose transporter gene hUGT1

• Horibe Takahiro

  Center for Gene Science, Hiroshima University

• Khalil Mohamed Farouk Mohamed

  Center for Gene Science, Hiroshima University

• Kawahara Tetsuya

  Center for Gene Science, Hiroshima University

• Ishida Nobuhiro

  Department of Environmental Security System, Faculty of Risk and Crisis Management, Chiba Institute of Science

• Tanaka Nobukazu

  Center for Gene Science, Hiroshima University

書誌事項

タイトル別名

• Modification of the surface carbohydrate composition of tobacco protoplasts transformed with the human UDP-galactose transporter gene hUGT1

抄録

Tobacco BY-2 cells were transformed with the human UDP-galactose transporter 1 gene (hUGT1) under the control of a 35S promoter. Accumulation of mRNA and protein derived from hUGT1 was detected in the hUGT1-transformed BY-2 cells. To identify the cell surface carbohydrates, BY-2 protoplasts were treated with 21 kinds of biotinylated lectin, stained with fluorescein isothiocyanate (FITC)-conjugated streptavidin and observed by fluorescence microscopy. FITC-ConA and -RCA₁₂₀ staining exhibited strong fluorescence on the surface of BY-2 protoplasts, whereas staining with other FITC-lectin conjugates showed faint or no fluorescence. Regarding FITC-ConA and -RCA₁₂₀ staining, the fluorescence of hUGT-expressing BY-2 protoplasts was weaker than that of control cells. Decreased FITC fluorescence of hUGT-expressing BY-2 protoplasts was also detected by fluorescence-activated cell sorting (FACS) analysis, suggesting that the surface carbohydrate composition was modified.

収録刊行物

• Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ

  Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 26 (4), 435-441, 2009

  日本植物バイオテクノロジー学会

キーワード

• BY-2
• FACS
• lectin
• tobacco
• UDP-galactose transporter

詳細情報

• CRID: 1390001204328482304
• NII論文ID: 10026136301
• NII書誌ID: AA11250821
• DOI: 10.5511/plantbiotechnology.26.435
• ISSN: 13476114
• ISSN: 13424580
• NDL書誌ID: 10539170
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10539170
• 本文言語コード: en
• データソース種別:
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