Modification of the surface carbohydrate composition of tobacco protoplasts transformed with the human UDP-galactose transporter gene hUGT1

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Abstract

Tobacco BY-2 cells were transformed with the human UDP-galactose transporter 1 gene (<i>hUGT1</i>) under the control of a 35S promoter. Accumulation of mRNA and protein derived from <i>hUGT1</i> was detected in the <i>hUGT1</i>-transformed BY-2 cells. To identify the cell surface carbohydrates, BY-2 protoplasts were treated with 21 kinds of biotinylated lectin, stained with fluorescein isothiocyanate (FITC)-conjugated streptavidin and observed by fluorescence microscopy. FITC-ConA and -RCA<sub>120</sub> staining exhibited strong fluorescence on the surface of BY-2 protoplasts, whereas staining with other FITC-lectin conjugates showed faint or no fluorescence. Regarding FITC-ConA and -RCA<sub>120</sub> staining, the fluorescence of <i>hUGT</i>-expressing BY-2 protoplasts was weaker than that of control cells. Decreased FITC fluorescence of <i>hUGT</i>-expressing BY-2 protoplasts was also detected by fluorescence-activated cell sorting (FACS) analysis, suggesting that the surface carbohydrate composition was modified.

Journal

  • Plant Biotechnology

    Plant Biotechnology 26(4), 435-441, 2009-09-25

    Japanese Society for Plant Cell and Molecular Biology

References:  23

Codes

  • NII Article ID (NAID)
    10026136301
  • NII NACSIS-CAT ID (NCID)
    AA11250821
  • Text Lang
    ENG
  • Article Type
    NOT
  • ISSN
    13424580
  • NDL Article ID
    10539170
  • NDL Source Classification
    ZR3(科学技術--生物学--植物)
  • NDL Call No.
    Z54-J126
  • Data Source
    CJP  NDL  J-STAGE 
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