Changes in the Levels of Progesterone Receptor mRNA and Protein in the Bovine Corpus Luteum During the Estrous Cycle

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Author(s)

Abstract

Progesterone (P4) is synthesized in the luteal cells of many species. The objective of the present study was to determine the expression pattern of P4 receptor (PR) mRNA and the distribution of PR protein in the bovine corpus luteum (CL) during the estrous cycle. The gene expression of <i>PR</i> in the bovine CL throughout the estrous cycle was determined by real-time PCR analysis, and the PR protein expression was evaluated by immunohistochemistry. Messenger RNA of <i>PR</i> was clearly expressed in the CL throughout the estrous cycle. The level of <i>PR</i> mRNA in the CL was highest at the early stage of the estrous cycle and was higher at the mid and late stages than at the regressed stage (P<0.01). In regard to the distribution of PR, the protein was expressed in both small and large luteal cells and in vascular endothelial cells throughout the estrous cycle. These results suggest that P4 has a role in regulating luteal and endothelial cell function in the bovine CL, especially at the early luteal stage as an autocrine/paracrine regulator.<br>

Journal

  • Journal of Reproduction and Development

    Journal of Reproduction and Development 56(2), 219-222, 2010-04-01

    THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

References:  22

Codes

  • NII Article ID (NAID)
    10026324376
  • NII NACSIS-CAT ID (NCID)
    AA10936678
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09168818
  • NDL Article ID
    10656472
  • NDL Source Classification
    ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z54-H305
  • DOI
    10.1262/jrd.09-141T
  • Data Source
    CJP  NDL  J-STAGE 
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