Expression and Localization of cFLIP, an Anti-apoptotic Factor, in the Bovine Corpus Luteum
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- Hojo Takuo HOJO Takuo
- Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
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- Al-Ziabi Mohamad Omar AL-ZL'ABI Mohamad Omar
- Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
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- Komiyama Junichi [他] KOMIYAMA Junichi
- Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
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- MANABE Noboru
- Research Unit for Animal Life Sciences, Animal Resource Science Center, The University of Tokyo
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- ACOSTA Tomas J
- Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
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- OKUDA Kiyoshi
- Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
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Author(s)
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- Hojo Takuo HOJO Takuo
- Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
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- Al-Ziabi Mohamad Omar AL-ZL'ABI Mohamad Omar
- Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
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- Komiyama Junichi [他] KOMIYAMA Junichi
- Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
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- MANABE Noboru
- Research Unit for Animal Life Sciences, Animal Resource Science Center, The University of Tokyo
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- ACOSTA Tomas J
- Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
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- OKUDA Kiyoshi
- Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
Abstract
The objective of the present study was to investigate the potential mechanisms regulating cellular FLICE-like inhibitory protein (cFLIP), an anti-apoptotic factor, in the bovine corpus luteum (CL). Expression of <i>cFLIP</i> mRNA was highest at the developing stage and then decreased significantly during the mid, late and regressed stages (P<0.05). Western blot analysis revealed that expression of the long isoform of cFLIP (cFLIP<sub>L</sub>) protein was high during the early and developing luteal stages, remained steady during the mid and late luteal stages and then decreased significantly (P<0.05) by the regressed stage. However, the expression levels of the short isoform of cFLIP (cFLIP<sub>S</sub>) remained low during the early, developing and mid luteal stages. Immunostaining of cFLIP was strongest in the cytoplasm of luteal and non-luteal cells, including endothelial and immune cells, remained high during the early, developing and mid luteal stages and then decreased significantly (P<0.05) in the late and regressed luteal stages. Immunostaining of cFLIP was observed only in macrophage-like cells in the regressing CL. However, cultured mid luteal cells had a higher percentage of cFLIP-positive cells and a lower percentage of TUNEL-positive cells than luteal cells treated with tumor necrosis factor α (TNF)/interferon γ (IFNG; P<0.01). These results indicate downregulation of cFLIP during structural luteal regression, suggesting that cFLIP plays a survival role in the bovine CL.<br>
Journal
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 56(2), 230-235, 2010-04-01
THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT
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