Expression and Localization of cFLIP, an Anti-apoptotic Factor, in the Bovine Corpus Luteum

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Author(s)

    • Hojo Takuo HOJO Takuo
    • Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
    • MANABE Noboru
    • Research Unit for Animal Life Sciences, Animal Resource Science Center, The University of Tokyo
    • ACOSTA Tomas J
    • Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
    • OKUDA Kiyoshi
    • Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University

Abstract

The objective of the present study was to investigate the potential mechanisms regulating cellular FLICE-like inhibitory protein (cFLIP), an anti-apoptotic factor, in the bovine corpus luteum (CL). Expression of <i>cFLIP</i> mRNA was highest at the developing stage and then decreased significantly during the mid, late and regressed stages (P<0.05). Western blot analysis revealed that expression of the long isoform of cFLIP (cFLIP<sub>L</sub>) protein was high during the early and developing luteal stages, remained steady during the mid and late luteal stages and then decreased significantly (P<0.05) by the regressed stage. However, the expression levels of the short isoform of cFLIP (cFLIP<sub>S</sub>) remained low during the early, developing and mid luteal stages. Immunostaining of cFLIP was strongest in the cytoplasm of luteal and non-luteal cells, including endothelial and immune cells, remained high during the early, developing and mid luteal stages and then decreased significantly (P<0.05) in the late and regressed luteal stages. Immunostaining of cFLIP was observed only in macrophage-like cells in the regressing CL. However, cultured mid luteal cells had a higher percentage of cFLIP-positive cells and a lower percentage of TUNEL-positive cells than luteal cells treated with tumor necrosis factor α (TNF)/interferon γ (IFNG; P<0.01). These results indicate downregulation of cFLIP during structural luteal regression, suggesting that cFLIP plays a survival role in the bovine CL.<br>

Journal

  • Journal of Reproduction and Development

    Journal of Reproduction and Development 56(2), 230-235, 2010-04-01

    THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

References:  37

Cited by:  2

Codes

  • NII Article ID (NAID)
    10026324441
  • NII NACSIS-CAT ID (NCID)
    AA10936678
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    09168818
  • NDL Article ID
    10656506
  • NDL Source Classification
    ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z54-H305
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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