Expression and Localization of cFLIP, an Anti-apoptotic Factor, in the Bovine Corpus Luteum
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- HOJO Takuo
- Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
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- AL-ZI'ABI Mohamad Omar
- Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
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- KOMIYAMA Junichi
- Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
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- MANABE Noboru
- Research Unit for Animal Life Sciences, Animal Resource Science Center, The University of Tokyo
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- ACOSTA Tomas J
- Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
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- OKUDA Kiyoshi
- Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
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Abstract
The objective of the present study was to investigate the potential mechanisms regulating cellular FLICE-like inhibitory protein (cFLIP), an anti-apoptotic factor, in the bovine corpus luteum (CL). Expression of cFLIP mRNA was highest at the developing stage and then decreased significantly during the mid, late and regressed stages (P<0.05). Western blot analysis revealed that expression of the long isoform of cFLIP (cFLIPL) protein was high during the early and developing luteal stages, remained steady during the mid and late luteal stages and then decreased significantly (P<0.05) by the regressed stage. However, the expression levels of the short isoform of cFLIP (cFLIPS) remained low during the early, developing and mid luteal stages. Immunostaining of cFLIP was strongest in the cytoplasm of luteal and non-luteal cells, including endothelial and immune cells, remained high during the early, developing and mid luteal stages and then decreased significantly (P<0.05) in the late and regressed luteal stages. Immunostaining of cFLIP was observed only in macrophage-like cells in the regressing CL. However, cultured mid luteal cells had a higher percentage of cFLIP-positive cells and a lower percentage of TUNEL-positive cells than luteal cells treated with tumor necrosis factor α (TNF)/interferon γ (IFNG; P<0.01). These results indicate downregulation of cFLIP during structural luteal regression, suggesting that cFLIP plays a survival role in the bovine CL.<br>
Journal
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 56 (2), 230-235, 2010
The Society for Reproduction and Development
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Keywords
Details 詳細情報について
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- CRID
- 1390282681314794880
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- NII Article ID
- 10026324441
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- NII Book ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL BIB ID
- 10656506
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed