Cationic Liposome Inhibits Gene Expression in an E.coliCell–Free Translation System

DOI Web Site Web Site 1 Citations 14 References
  • Thi Bui Huong
    Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University
  • Umakoshi Hiroshi
    Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University
  • Suga Keishi
    Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University
  • Tanabe Tomoyuki
    Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University
  • Shimanouchi Toshinori
    Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University
  • Kuboi Ryoichi
    Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University

Search this article

Abstract

The role of liposome addition on the gene expression of green fluorescent protein (GFP)was studied in an in vitroprotein translation system in the presence of cationic liposome, prepared by 1,2–dioleoyl–3–trimethyl–ammonium–propane (DOTAP). GFP fluorescence after gene expression was reduced with an increase in DOTAP concentrations,and no fluorescence was observed at certain concentrations of DOTAP. It was found that neither translated GFP nortranscript mRNA for GFP were produced at the above conditions. The above results may show that the DOTAP lipo-some canaffect the inhibition at the transcription step of the GFP synthesis process in addition to its translation andfolding steps.

Journal

  • MEMBRANE

    MEMBRANE 34 (3), 146-151, 2009

    THE MEMBRANE SOCIETY OF JAPAN

Citations (1)*help

See more

References(14)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top