Effects of In Vitro Aging of Mouse Oocytes on Metaphase II Spindle Morphology, In Vitro Fertilization and Subsequent Embryonic Development

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  • Takahashi Kazumasa
    United Graduate School of Agriculture, Tokyo University of Agriculture and Technology Central Clinic
  • Matsui Hitomi
    Laboratory of Animal Breeding and Reproduction, Faculty of Agriculture, Utsunomiya University
  • Takahashi Ikue
    Laboratory of Animal Breeding and Reproduction, Faculty of Agriculture, Utsunomiya University
  • Matsumoto Hiromichi
    United Graduate School of Agriculture, Tokyo University of Agriculture and Technology Laboratory of Animal Breeding and Reproduction, Faculty of Agriculture, Utsunomiya University
  • Fukui Emiko
    United Graduate School of Agriculture, Tokyo University of Agriculture and Technology Laboratory of Animal Breeding and Reproduction, Faculty of Agriculture, Utsunomiya University
  • Motoyama Mitsuhiro
    Central Clinic
  • Yoshizawa Midori
    United Graduate School of Agriculture, Tokyo University of Agriculture and Technology Laboratory of Animal Breeding and Reproduction, Faculty of Agriculture, Utsunomiya University

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Other Title
  • マウス卵子の第2減数分裂中期紡錘体の形態、体外受精および胚発生に対するin vitro agingの影響
  • Effects of in vitro aging of mouse oocytes on metaphase 2 spindle morphology, in vitro fertilization and subsequent embryonic development

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Abstract

The aim of the present study was to reveal the effects of in vitro aging of mouse oocytes without cumulus cells on morphological alteration of their metaphase II (MII) spindles, ability to be fertilized in vitro and the developmental competence of the resulting embryos. To observe the MII spindles by immunofluorescence staining, oocytes without cumulus cells were divided into four groups: a non-aged control and three others aged for 10, 15 or 25 h. Although the incidence of morphologically normal spindles was significantly lower in the 25 h group, no significant increase of chromosome misalignment was observed in any of the aged groups (P < 0.05). For the in vitro fertilization (IVF) experiments, denuded oocytes were aged for 15, 16.5 or 18 h and the resulting normal zygotes with a second polar body were cultured in vitro for 120 h to assess their embryonic development. The fertilization rate was significantly lower only in the 18-h aged group. The rates of blastocyst formation were significantly lower in all aged oocyte groups, compared with non-aged controls, and blastocysts derived from aged oocytes had lower total cell numbers. Therefore, a significant decline of developmental competence appears in cumulus cell-removed oocytes aged for more than 15 h, even if they retain fertilizability.<br>

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