Metabolic profiling analysis of genetically modified rice seedlings that overproduce tryptophan reveals the occurrence of its inter-tissue translocation

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A metabolic profiling approach using high performance liquid chromatography-mass spectrometry (LC-MS) was applied to seedlings of transgenic rice plants (<i>Oryza sativa</i>) that over-express the <i>OASA1D</i> gene encoding a feedback-insensitive α-subunit of anthranilate synthase (AS, EC 4.1.3.27). Analysis revealed that the seedlings accumulated tryptophan (Trp) at a high concentration without marked effects on the amounts of other major metabolites. Some minor indole metabolites showed a certain degree of increase in the amounts in Trp-accumulating tissues, while no active catabolic conversion of Trp was indicated. Analysis also revealed that the distribution of Trp in the plant was uneven, with the highest level being observed in young developing tissues, despite tissue-independent expression of the <i>OASA1D</i> gene under the control of ubiquitin promoter. Differences in AS activity and anthranilate content among organs were small. A feeding experiment with radiolabeled Trp clearly demonstrated one-way Trp movement from old to young leaves; thus, the uneven distribution of Trp in <i>OASA1D</i> plants is the manifestation of Trp translocation. The negligible effects of Trp accumulation in other metabolic pathways, low metabolic activity of Trp, and efficient translocation of Trp in rice plants expressing <i>OASA1D</i> transgene are favorable characters from the aspect of metabolic engineering of Trp production.

収録刊行物

  • Plant biotechnology

    Plant biotechnology 27(1), 17-27, 2010-03-25

    日本植物細胞分子生物学会

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各種コード

  • NII論文ID(NAID)
    10026411273
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13424580
  • NDL 記事登録ID
    10606941
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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