Isolation of multidrug resistance associated protein like gene from lead hyperaccumulator buckwheat and its lead detoxification ability
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- Mizuno Takafumi
- Graduate School of Bioresources, Mie University
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- Nakagawa Masahiro
- Graduate School of Bioresources, Mie University
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- Ono Hiroshi
- Graduate School of Bioresources, Mie University
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- Sugiura Daisuke
- Graduate School of Bioresources, Mie University
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- Tamura Hideo
- Biotechnology Group, Energy Application R&D Center, Chubu Electric Power Co.
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- Obata Hitoshi
- Graduate School of Bioresources, Mie University
書誌事項
- タイトル別名
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- Isolation of multidrug resistance associated protein like gene from lead hyperaccumulator common buckwheat and its lead detoxification ability
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A multidrug resistance associated protein homolog gene, named FeABCC1, was isolated from a commercial brand of common buckwheat that has high lead tolerance and accumulation ability. The deduced amino acid sequence of FeABCC1 had 64% identity with AtMRP3 (AtABCC3) of Arabidopsis thaliana, and was predicted as integral membrane protein with 12 transmembrane domains. FeABCC1 expression was induced in the shoot of common buckwheat treated with lead. The yeast transformed with FeABCC1 showed a marked increasing in lead tolerance with the gene expression, and accumulated more lead compared to control. Not similar to AtMRP3, FeABCC1 expression did not effect to cadmium tolerance in yeast, and inhibition of glutathione synthesis by buthionine sulfoximine was not effect lead tolerance. These data suggests that FeABCC1 has some role for lead detoxyfication in common buckwheat independently to glutathione chelating.
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 27 (1), 39-46, 2010
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390001204327235456
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- NII論文ID
- 10026411374
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- NII書誌ID
- AA11250821
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 10606979
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可