Tissue culture note: Triterpenoid levels are reduced during Euphorbia tirucalli L. callus formation

• Uchida Hidenobu

  School of Environmental Science and Engineering, Kochi University of Technology

• Ohyama Kiyoshi

  Department of Chemistry and Materials Science, Tokyo Institute of Technology Plant Science Center, RIKEN

• Suzuki Masashi

  Plant Science Center, RIKEN

• Yamashita Hirofumi

  Graduate School of Human and Environmental Sciences, Kyoto Prefectural University

• Muranaka Toshiya

  Plant Science Center, RIKEN Kihara Institute for Biological Research, Yokohama City University

• Ohyama Kanji

  Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University

書誌事項

タイトル別名

• Triterpenoid levels are reduced during Euphorbia tirucalli L. callus formation

抄録

In order to compare the profiles of hydrophobic secondary metabolites between the calli and plants of Euphorbia tirucalli, we analyzed their free sterol and free triterpenoid contents by GC-MS. We obtained the calli by culturing E. tirucalli internodes successively on solidified B5 medium containing hormones. The aerial parts of the plants or calli were extracted with CHCl₃-MeOH (2 : 1, v/v) and subjected to GC-MS analysis. In a plant extract, only two sterol peaks, for campesterol and β-sitosterol, and at least 11 peaks of the total ion chromatogram (TIC) with a [M⁺] of 426, including peaks for euphol, β-amyrin, and glutinol, were detected. The ratio of triterpenoid- and triterpenoid-like-peaks of TIC with a [M⁺] of 426 to the total of all peaks detected was ca. 86% (n=3) in the plant extract. In a callus extract, 6 sterols, campesterol, stigmasterol, β-sitosterol, isofucosterol, cycloartenol, and 24-methylenecycloartanol, and two triterpenoids with a molecular weight of 426 (euphol and β-amyrin) were detected. The ratio of triterpenoid- and triterpenoid-like peaks of TIC with a [M⁺] of 426 to the total of all peaks detected was only ca. 6.3% (n=3) in the callus extract. These results indicate that the ratio of free triterpenoids and free triterpenoid-like compounds (MW: 426) to free sterols is lower in the calli than in the plants of E. tirucalli.

収録刊行物

• Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ

  Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 27 (1), 105-109, 2010

  日本植物バイオテクノロジー学会

キーワード

• Callus
• <i>Euphorbia tirucalli</i>
• petroleum plant
• sterol
• triterpenoid

詳細情報

• CRID: 1390001204327242240
• NII論文ID: 10026411583
• NII書誌ID: AA11250821
• DOI: 10.5511/plantbiotechnology.27.105
• ISSN: 13476114
• ISSN: 13424580
• NDL書誌ID: 10607126
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10607126
• 本文言語コード: en
• データソース種別:
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  • NDL
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  • CiNii Articles
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