Co-occurrence of DNA- and RNA-viruses infecting the bloom-forming dinoflagellate, <i>Heterocapsa circularisquama</i>, on the Japan coast

• Tomaru Yuji

  Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency

• Mizumoto Hiroyuki

  Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency

• Takao Yoshitake

  Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency

• Nagasaki Keizo

  Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency

Bibliographic Information

Other Title

• Co-occurrence of DNA- and RNA-viruses infecting the bloom-forming dinoflagellate, Heterocapsa circularisquama, on the Japan coast

Abstract

In western Japan, Heterocapsa circularisquama blooms occur almost every year. Two distinct viruses infectious to H. circularisquama, a large double-stranded DNA (dsDNA) virus, ‘HcV’, and a small single-stranded RNA (ssRNA) virus, ‘HcRNAV’, were found and characterized. Here we report the co-occurrence of the two viruses in Hiroshima Bay, Japan, during 2006. Viruses isolated using H. circularisquama strains HU9433-P and MZ2 (the hosts) were large dsDNA viruses (ca. 0.2 μm in diameter) that accumulated in the host cytoplasm. Pulsed-field gel electrophoresis analysis showed their genome sizes were ca. 388 kbp, comparable to the previously reported HcV strain. Small ssRNA viruses were also isolated (using the host strain HCLG-1) at ca. 30 nm in diameter and formed crystalline arrays and/or were randomly accumulated in the host cytoplasm, and their partial genome sequences were highly similar to a previously reported HcRNAV strain. This is the first report of the co-occurrence of HcV and HcRNAV in natural water and their successful isolation. The data suggest the two distinct viruses may cooperatively affect the dynamics of H. circularisquama blooms.

Journal

• Plankton and Benthos Research

  Plankton and Benthos Research 4 (4), 129-134, 2009

  The Plankton Society of Japan, The Japanese Association of Benthology

Keywords

• Algal virus
• dinoflagellate
• HcRNAV
• HcV
• <i>Heterocapsa circularisquama</i>

Details

• CRID: 1390282680241995520
• NII Article ID: 10026447256
• NII Book ID: AA12130745
• DOI: 10.3800/pbr.4.129
• ISSN: 1882627X
• ISSN: 18808247
• NDL BIB ID: 10457361
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10457361
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed