The effect of ethanol fixation on stable isotope signatures in benthic organisms
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- Yoshino Kenji YOSHINO KENJI
- Ariake Sea Research Project, Saga University
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- Amano Yoshimasa AMANO YOSHIMASA
- Ariake Sea Research Project, Saga University
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- Fukumori Kayoko [他] FUKUMORI KAYOKO
- Center for Marine Environmental Studies, Ehime University
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- HAMAOKA HIDEKI
- Center for Marine Environmental Studies, Ehime University
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- MIYASAKA HITOSHI
- Center for Marine Environmental Studies, Ehime University
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- OMORI KOJI
- Center for Marine Environmental Studies, Ehime University
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Author(s)
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- Yoshino Kenji YOSHINO KENJI
- Ariake Sea Research Project, Saga University
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- Amano Yoshimasa AMANO YOSHIMASA
- Ariake Sea Research Project, Saga University
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- Fukumori Kayoko [他] FUKUMORI KAYOKO
- Center for Marine Environmental Studies, Ehime University
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- HAMAOKA HIDEKI
- Center for Marine Environmental Studies, Ehime University
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- MIYASAKA HITOSHI
- Center for Marine Environmental Studies, Ehime University
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- OMORI KOJI
- Center for Marine Environmental Studies, Ehime University
Abstract
Stable isotope analyses are often used for the estimation of food web structures based on empirical fractionation (i.e., enrichment of carbon isotope signatures at 0–1‰ with one trophic level, and that of nitrogen at 3–4‰). We examined the effect of 80% ethanol fixation on both carbon and nitrogen stable isotope signatures of six species of macrobenthos samples. The effect of fixation was inconsistent (i.e. both enrichment and depletion occurred). The shift of nitrogen isotope signatures due to fixation ranged from −0.3 to +0.8‰, and the carbon signatures of fixed samples showed enrichment by −0.3 to +1.3‰ depending on the species. These changes would clearly affect estimation of trophic levels and energy flow in food webs. Ethanol fixation may not be a smart choice because of the inconsistency of the effect on isotope signatures. However, if the effect was corrected for each species, ethanol-fixed samples can be practically useful for food web analyses using stable isotopes.
Journal
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- Plankton and Benthos Research
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Plankton and Benthos Research 5(2), 79-82, 2010-05-01
The Plankton Society of Japan, The Japanese Association of Benthology
References: 15
-
1
- δ^<13>C measurements as indicators of carbon flow in marine and freshwater ecosystems
-
FRY B
Contrib Mar Sci 27, 13-47, 1984
Cited by (1)
-
2
- Stable isotopes show food web changes after invasion by the predatory cladoceran Cercopagis pengoi in a Baltic Sea bay
-
GOROKHOVA E
Oecologia 143, 251-259, 2005
Cited by (1)
-
3
- Preservation of blood and tissue samples for stable-carbon and stable nitrogen isotope analysis
-
HOBSON KA
Can J Zool 75, 1720-1723, 1997
Cited by (1)
-
4
- The effects of preservation on fish tissue stable isotope signatures
-
KELLY B
J Fish Biol 69, 1595-1611, 2006
Cited by (1)
-
5
- Stable nitrogen isotopes in zooplankton : some geographic and temporal variations in the North Pacific
-
MULLIN MM
Limnol Oceanogr 29, 1267-1273, 1984
Cited by (1)
-
6
- Uncertainty in source partitioning using stable isotopes
-
PHILLIPS DL
Oecologia 127, 171-179, 2001
Cited by (1)
-
7
- Effects of several preservation methods on the isotopic content of Drosophia samples
-
PONSARD S
C R Acad Sci Paris Sci Vie 322, 35-41, 1999
Cited by (1)
-
8
- A synthesis of tissue-preservation effects on carbon and nitrogen stable isotope signatures
-
SARAKINOS HC
Can J Zool 80, 381-387, 2002
Cited by (1)
-
9
- Using stable isotopes to quantify material transport in food webs
-
SCHINDLER DE
Food Webs at the Landscape Level, 25-42, 2004
Cited by (1)
-
10
- Preservation effects on stable isotope analysis of fish muscle
-
ARRINGTON DA
Trans Amer Fish Soc 131, 337-342, 2002
Cited by (1)
-
11
- Mechanism of carbon isotope fractionation associated with lipid synthesis
-
DENIRO M. J.
Science 197, 261-263, 1977
DOI Cited by (6)
-
12
- Effects of storage and preservation on the δ^<13>C and δ^<15>N signatures of selected marine organisms
-
KAEHLER S
Mar Ecol Prog Ser 219, 299-304, 2001
Cited by (1)
-
13
- <no title>
-
POST DM
Ecology 83, 703-718, 2002
DOI Cited by (2)
-
14
- Tissue and fixative dependent shifts of δ^<13>C and d15N in preserved ecological material
-
SWEETING CJ
Rapid Commun Mass Spectrom 18, 2587-2592, 2004
Cited by (1)
-
15
- Historical food web structure and the restoration of native aquatic communities in the Lake Tahoe (California-Nevada) basin
-
VANDER ZANDEN MJ
Ecosystems 6, 274-288, 2003
Cited by (3)