Proliferation and differentiation potential of pluripotent mesenchymal precursor C2C12 cells on resin-based restorative materials

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Author(s)

    • IMAZATO Satoshi
    • Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry
    • HORIKAWA Daisuke
    • Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry
    • TAKEDA Kahoru
    • Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry
    • KIBA Wakako
    • Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry
    • IZUTANI Naomi
    • Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry
    • YOSHIKAWA Ranna
    • Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry
    • HAYASHI Mikako
    • Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry
    • EBISU Shigeyuki
    • Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry
    • NAKANO Takayoshi
    • Department of Materials Science and Engineering, Osaka University Graduate School of Engineering

Abstract

This study investigated the proliferation and differentiation potential of pluripotent mesenchymal cells on three resin-based restoratives using a typical pluripotent mesenchymal precursor cell line, C2C12. C2C12 cells were cultured for 3-21 days on cured specimens of a Bis-GMA/TEGDMA-based composite resin (APX; Clearfil AP-X), a 4-META/MMA-based resin cement (SB; Superbond C&B) or a HEMA-containing resin modified glass-ionomer (LC; Fuji Ionomer Type II LC). To examine the influences on differentiation potential, alkaline phosphatase (ALP) activity of the cells cultured on each material was determined. On APX and SB, cells adhered and proliferated well, and no significant influences on ALP activity were observed. In contrast, poor cell proliferation and significant suppression of ALP activity were observed for cells cultured on LC, similar to those cultured on a zinc oxide EBA cement used as a control material. Bis-GMA/TEGDMA-based composite resin and 4-META/MMA-based resin exhibited better biocompatibility for C2C12 cells than HEMA-containing resin modified glass-ionomer, suggesting a potential advantage of the former two resins to show smaller influences on regeneration of periapical or periodontal tissue.

Journal

  • Dental Materials Journal

    Dental Materials Journal 29(3), 341-346, 2010-05-01

    The Japanese Society for Dental Materials and Devices

References:  33

Cited by:  1

Codes

  • NII Article ID (NAID)
    10026470432
  • NII NACSIS-CAT ID (NCID)
    AA10443149
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    02874547
  • Data Source
    CJP  CJPref  J-STAGE 
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