Proliferation and differentiation potential of pluripotent mesenchymal precursor C2C12 cells on resin-based restorative materials

• IMAZATO Satoshi

  Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry

• HORIKAWA Daisuke

  Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry

• TAKEDA Kahoru

  Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry

• KIBA Wakako

  Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry

• IZUTANI Naomi

  Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry

• YOSHIKAWA Ranna

  Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry

• HAYASHI Mikako

  Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry

• EBISU Shigeyuki

  Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry

• NAKANO Takayoshi

  Department of Materials Science and Engineering, Osaka University Graduate School of Engineering

Abstract

This study investigated the proliferation and differentiation potential of pluripotent mesenchymal cells on three resin-based restoratives using a typical pluripotent mesenchymal precursor cell line, C2C12. C2C12 cells were cultured for 3-21 days on cured specimens of a Bis-GMA/TEGDMA-based composite resin (APX; Clearfil AP-X), a 4-META/MMA-based resin cement (SB; Superbond C&B) or a HEMA-containing resin modified glass-ionomer (LC; Fuji Ionomer Type II LC). To examine the influences on differentiation potential, alkaline phosphatase (ALP) activity of the cells cultured on each material was determined. On APX and SB, cells adhered and proliferated well, and no significant influences on ALP activity were observed. In contrast, poor cell proliferation and significant suppression of ALP activity were observed for cells cultured on LC, similar to those cultured on a zinc oxide EBA cement used as a control material. Bis-GMA/TEGDMA-based composite resin and 4-META/MMA-based resin exhibited better biocompatibility for C2C12 cells than HEMA-containing resin modified glass-ionomer, suggesting a potential advantage of the former two resins to show smaller influences on regeneration of periapical or periodontal tissue.

Journal

• Dental Materials Journal

  Dental Materials Journal 29 (3), 341-346, 2010

  The Japanese Society for Dental Materials and Devices

Keywords

• Restorative materials
• Resins
• Mesenchymal cells

Details

• CRID: 1390282679693817600
• NII Article ID: 10026470432
• NII Book ID: AA10443149
• DOI: 10.4012/dmj.2009-082
• ISSN: 18811361
• ISSN: 02874547
• Web Site: https://www.jstage.jst.go.jp/article/dmj/29/3/29_2009-082/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2011052628
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed