液体クロマトグラフィー/質量分析法を用いる環境水中副腎皮質ステロイド類分析法の開発  [in Japanese] Determination of Corticosteroids in Environmental Water by Liquid Chromatography/Mass Spectrometry  [in Japanese]

Access this Article

Search this Article

Author(s)

Abstract

環境試料中に含まれる副腎皮質ステロイド類の液体クロマトグラフィー/質量分析(LC/MS)法を確立し,河川水に適用した.測定対象とした副腎皮質ステロイドは,プレドニゾロン,デキサメタゾン,ベタメタゾン,トリアムシノロンアセトニド,フルオシノロンアセトニド,酢酸プレドニゾロン,酢酸ヒドロコルチゾン,吉草酸ベタメタゾン及び二プロピオン酸ベタメタゾンの9種である.採水した河川水はガラス繊維ろ紙でろ過した後,ろ液からの測定対象物質の抽出及び濃縮を固相抽出法で行った.溶出にはアセトニトリル5 mLを用い,この溶液を濃縮し,アセトニトリルで溶解して1 mLとしたものをLC/MSに注入した.移動相はアセトニトリル-酢酸アンモニウム緩衝液を用いてグラジェント溶離し,MSで検出した.最適条件下,作成した各測定対象物質の検量線は相関係数0.9971以上の直線性を示し,検出限界は<i>S</i>/<i>N</i>=3とした時0.7~2.4 ng/Lであった.また,本測定法を河川水に適用した結果,10 ng/Lの添加回収試験において回収率は56.2~110%であった.

The determination of 9 corticosteroids in environment samples by a liquid chromatography/mass spectrometry (LC/MS) was established, and was applied to river water. After river-water samples were filtered through a glass microfiber filter, GF/B, these filtrates were passed through a solid-phase extraction cartridge and extracted corticosteroids. Extraction solutions were concentrated and re-fused by acetonitrile, and the resulting extracts were directly injected into the LC/MS system. The mobile phase used was acetonitrile - ammonium acetate buffer (pH 5.0) for gradient elution and detected by MS. Under the optimum analytical conditions, the correlation coefficients of calibration curves were more than 0.9971, and the limits of detection (<i>S</i>/<i>N</i> = 3) were in the range of 0.7∼2.4 ng/L. The recoveries of corticosteroids from river water spiked at 10 ng/L were 56.2∼110%. This method was satisfactorily used for the determination of corticosteroids in river-water samples.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 59(4), 301-309, 2010-04-05

    The Japan Society for Analytical Chemistry

References:  15

  • <no title>

    ALDA M. J. L.

    J. Chromatogr. A 1000, 503, 2003

    Cited by (1)

  • <no title>

    TERNES T. A.

    Water Res. 32(11), 3245, 1998

    Cited by (1)

  • <no title>

    TERNES T.

    J. Chromatogr. A 938, 175, 2001

    Cited by (1)

  • <no title>

    HEBERER T.

    J. Hydrol. 266, 175, 2002

    Cited by (1)

  • <no title>

    KOUTSOUBA V.

    Chemosphere 51, 69, 2003

    Cited by (1)

  • <no title>

    STRAUB J. O.

    Toxicol. Lett. 131, 137, 2002

    Cited by (1)

  • <no title>

    JONES H.

    Water Res. 36, 5013, 2002

    Cited by (1)

  • <no title>

    石井善昭

    環境化学 14(1), 127, 2004

    Cited by (1)

  • <no title>

    清野敦子

    水環境学会誌 27(11), 685, 2004

    Cited by (1)

  • <no title>

    清野敦子

    用水と廃水 47(2), 39, 2005

    Cited by (1)

  • <no title>

    厚生労働省医政局

    薬事工業生産動態統計年報平成14年, 2003

    Cited by (1)

  • <no title>

    三田村邦子

    分析化学 (Bunseki Kagaku) 48, 401, 1999

    Cited by (1)

  • <no title>

    中村洋

    液クロ 彪の巻 127, 2004

    Cited by (1)

  • <no title>

    BUDAVARI Susan Ed.

    The Merck Index, An Encyclopedia of Chemicals, Drugs, and Biologicals, 1996

    Cited by (1)

  • <no title>

    SNYDER S. A.

    Environmental Engineering Science 20(5), 449-469, 2003

    DOI Cited by (3)

Cited by:  1

Codes

  • NII Article ID (NAID)
    10026475930
  • NII NACSIS-CAT ID (NCID)
    AN00222633
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    05251931
  • NDL Article ID
    10667074
  • NDL Source Classification
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL Call No.
    Z17-9
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
Page Top