Ectopic expression of an AP3-like and a PI-like genes from 'Sekkoku' orchid (Dendrobium moniliforme) causes the homeotic conversion of sepals to petals in whorl 1 and the suppression of carpel development in whorl 4 in Arabidopsis flowers
-
- Sirisawat Supatida SIRISAWAT Supatida
- Graduate School of Life and Environmental Science, University of Tsukuba
-
- Ezura Hiroshi EZURA Hiroshi
- Graduate School of Life and Environmental Science, University of Tsukuba
-
- Fukuda Naoya [他] FUKUDA Naoya
- Graduate School of Life and Environmental Science, University of Tsukuba
-
- KOUNOSU Takahiro
- Graduate School of Life and Environmental Science, University of Tsukuba
-
- HANDA Takashi
- School of Agriculture, Meiji University
Access this Article
Search this Article
Author(s)
-
- Sirisawat Supatida SIRISAWAT Supatida
- Graduate School of Life and Environmental Science, University of Tsukuba
-
- Ezura Hiroshi EZURA Hiroshi
- Graduate School of Life and Environmental Science, University of Tsukuba
-
- Fukuda Naoya [他] FUKUDA Naoya
- Graduate School of Life and Environmental Science, University of Tsukuba
-
- KOUNOSU Takahiro
- Graduate School of Life and Environmental Science, University of Tsukuba
-
- HANDA Takashi
- School of Agriculture, Meiji University
Abstract
Three class-B MADS-box genes, the paleotype <i>AP3</i> genes <i>DMAP3A</i> and <i>DMAP3B,</i> and the <i>PI</i>-like gene <i>DMPI</i>, were isolated from a native orchid in Japan named ‘Sekkoku’ (<i>Dendrobium moniliforme</i>) and ectopically expressed in <i>Arabidopsis</i>. <i>DMAP3A</i> and <i>DMPI</i> were expressed in all four floral whorls in the orchid, whereas <i>DMAP3B</i> was expressed only in whorls 2, 3, and 4. The ectopic expression of 35S::<i>DMPI</i> caused a partial sepal-to-petal conversion in whorl 1, increased the longevity of the flowers and delayed silique maturation in the transgenic plants, compared with wild-type plants. Transgenic <i>Arabidopsis</i> plants over-expressing 35S::<i>DMAP3B/</i>35S::<i>DMPI</i> exhibited a vegetative phenotype with leaf curling, a near-complete sepal-to-petal conversion in whorl 1, and the suppression of carpel development in whorl 4. Our results indicate that <i>DMAP3B</i> and <i>DMPI</i> are major class B MADS-box genes in <i>D. moniliforme</i> and may play an important role in the development of petals in the second floral whorl of the orchid. In addition, our results also suggest that these genes can be used for the genetic engineering of flower shape in plants.
Journal
-
- Plant Biotechnology
-
Plant Biotechnology 27(2), 183-192, 2010-06-25
Japanese Society for Plant Cell and Molecular Biology
References: 43
-
1
- Functional diversification of B MADS-box homeotic regulators of flower development : adaptive evolution in protein-protein interaction domains after major gene duplication events
-
HERNANDEZ-HERNANDEZ T
Mol Biol Evol 24, 465-481, 2007
Cited by (1)
-
2
- Structural and functional analysis of rose class B MADS-box genes 'MASAKO BP, euAP3, and B3': Paleotype AP3 homologue 'MASAKO B3' association with petal development
-
HIBINO Y
Plant Sci 170, 778-785, 2006
Cited by (1)
-
3
- Molecular evolution of genes controlling petal and stamen development : duplication and divergence within the APETALA3 and PISTILLATA MADS-box gene lineages
-
KRAMER EM
Genetics 149, 765-783, 1998
Cited by (1)
-
4
- The Arabidopsis homeotic genes APETALA3 and PISTILLATA are sufficient to provide the B class organ identity function
-
KRIZEK BA
Development 122, 11-22, 1996
Cited by (1)
-
5
- Positive selection and ancient duplications in the evolution of class B floral homeotic genes of orchids and grasses
-
MONDRAGON-PALOMINO M
BMC Evol Biol 21, 9:81, 2009
Cited by (1)
-
6
- MADS about the evolution of orchid flowers
-
MONDRAGON-PALOMINO M
Trends Plant Sci 13, 51-59, 2008
Cited by (1)
-
7
- The MADS box gene family in tomato : temporal expression during floral development, conserved secondary structures and homology with homeotic genes from Antirrhinum and Arabidopsis
-
PNUELI L
Plant J 1, 255-266, 1991
Cited by (1)
-
8
- DMMADS4, a DEF-like gene from Dendrobium is required for floral organ identity and flower longevity of orchid
-
SIRISAWAT S
Acta Hart 836, 259-264, 2009
Cited by (1)
-
9
- The MIK region rather than the C-terminal domain of AP3-like class B floral homeotic proteins determines functional specificity in the development and evolution of petals
-
SU K
New Phytol 178, 544-558, 2008
Cited by (1)
-
10
- Floral organogenesis in Tulipa
-
VAN TUNEN AJ
Flowering Newsl 16, 33-38, 1993
Cited by (1)
-
11
- Evolution of class B floral homeotic proteins : obligate heterodimerization originated from homodimerization
-
WINTER KU
Mol Biol Evol 19, 587-596, 2002
Cited by (1)
-
12
- Organ identity genes and modified patterns of flower development in Gerbera hybrid (Asteraceae)
-
YU D
Plant J 17, 51-62, 1999
Cited by (1)
-
13
- Ovary and gametophyte development are coordinately regulated by auxin and ethylene following pollination
-
ZHANG XS
Plant Cell 5, 403-418, 1993
Cited by (1)
-
14
- Molecular and genetic analyses of the silky gene reveal conservation in floral organ specification between eudicots and monocots
-
AMBROSE B. A.
Mol Cell 5, 569-579, 2000
DOI Cited by (7)
-
15
- Evolutionary conservation of angiosperm flower development at the molecular and genetic levels
-
BOWMAN J. L.
J Biosci 22, 515-527, 1997
DOI Cited by (5)
-
16
- Floral dip : a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
-
CLOUGH S. J.
Plant J. 16, 735-743, 1998
DOI Cited by (85)
-
17
- The war of the whorls : genetic interactions controlling flower development
-
COEN E. S.
Nature 353, 31-37, 1991
Cited by (59)
-
18
- Function and regulation of the Arabidopsis floral homeotic gene PISTILLATA
-
GOTO K.
Genes Dev. 8, 1548-1560, 1994
DOI Cited by (31)
-
19
- An Orchid (Oncidium Gower Ramsey) AP3-like MADS Gene Regulates Floral Formation and Initiation
-
HSU Hsing-Fun , YANG Chang-Hsien
Plant and Cell Physiology 43(10), 1198-1209, 2002-10-01
References (59) Cited by (11)
-
20
- Arabidopsis homeotic gene APETALA3 ectopic expression : transcriptional and post-transcriptional regulation determine floral organ identity
-
JACK T.
Cell 76, 703-716, 1994
DOI Cited by (8)
-
21
- Heterotopic expression of class B floral homeotic genes supports a modified ABC model for tulip (Tulipa gesneriana)
-
KANNO A.
Plant Mol Biol 52, 831-841, 2003
DOI Cited by (11)
-
22
- Expression of floral MADS-box genes in basal angiosperms : implications for the evolution of floral regulators
-
KIM S
Plant J 43, 724-744, 2005
DOI Cited by (3)
-
23
- Expression of a DEFICIENS-like gene correlates with the differentiation between sepal and petal in the orchid, Habenaria radiata (Orchidaceae)
-
KIM S. Y.
Plant Sci. 172, 319-326, 2007
Cited by (5)
-
24
- Evolution of genetic mechanisms controlling petal development
-
KRAMER E. M.
Nature 399, 144-148, 1999
Cited by (3)
-
25
- Evolution of the petal and stamen developmental programs : evidence from comparative studies of the lower eudicots and basal angiosperms
-
KRAMER EM
Int J Plant Sci 161, S29-S40, 2000
DOI Cited by (5)
-
26
- Functional divergence within the APETALA3/PISTILLATA floral homeotic gene lineages
-
LAMB R. S.
Proc. Natl. Acad. Sci. USA 100, 6558-6563, 2003
Cited by (5)
-
27
- Reconstitution of 'floral quartets' in vitro involving class B and class E floral homeotic proteins
-
MELZER R
Nucleic Acids Res 37, 2723-2736, 2009
Cited by (1)
-
28
- Identification of a rice APETALA3 homologue by yeast two-hybrid screening
-
MOON Y. -H.
Plant Mol Biol 40, 167-177, 1999
Cited by (13)
-
29
- Ovule development: identification of stage specific and tissue specific cDNAs.
-
NADEAU J. A.
Plant Cell 8, 213-239, 1996
Cited by (8)
-
30
- Two GLOBOSA-Like Genes are Expressed in Second and Third Whorls of Homochlamydeous Flowers in Asparagus officinalis L.
-
PARK Jin-Heui , ISHIKAWA Yuichi , OCHIAI Toshinori , KANNO Akira , KAMEYA Toshiaki
Plant and Cell Physiology 45(3), 325-332, 2004-03-01
References (29) Cited by (8)
-
31
- Characterization of the Antirrhinum floral homeotic MADS-box gene deficiens : evidence for DNA binding and autoregulation of its persistent expression throughout flower development
-
SCHWARZ-SOMMER Z.
EMBO J 11, 251-263, 1992
Cited by (10)
-
32
- The MADS-box family of transcription factors
-
SHORE P.
Eur. J. Biochem. 229, 1-13, 1995
DOI Cited by (15)
-
33
- Cloning and transcription analysis of an AGAMOUS- and SEEDSTICK ortholog in the orchid Dendrobium thyrsiflorum (Reichb. f.)
-
SKIPPER M.
Gene 366, 266-274, 2006
Cited by (4)
-
34
- Classification and phylogeny of the MADS-box multigene family suggest defined roles of MADS-box gene subfamilies in the morphological evolution of eukaryotes
-
Theiβan G.
J Mol Evol 43, 484-516, 1996
Cited by (20)
-
35
- GLOBOSA : A homeotic gene which interacts with DEFICIENS in the control of Antirrhinum floral organogenesis
-
TROBNER W.
EMBO J. 11, 4693-4704, 1992
Cited by (21)
-
36
- Four DEF-Like MADS Box Genes Displayed Distinct Floral Morphogenetic Roles in Phalaenopsis Orchid
-
TSAI Wen-Chieh , KUOH Chang-Sheng , CHUANG Ming-Hsiang , CHEN Wen-Huei , CHEN Hong-Hwa
Plant and Cell Physiology 45(7), 831-844, 2004-07-01
References (35) Cited by (11)
-
37
- PeMADS6, a GLOBOSA/PISTILLATA-like Gene in Phalaenopsis equestris Involved in Petaloid Formation, and Correlated with Flower Longevity and Ovary Development
-
TSAI Wen-Chieh , LEE Pei-Fang , CHEN Hong-Ie , HSIAO Yu-Yun , WEI Wan-Ju , PAN Zhao-Jun , CHUANG Ming-Hsiang , KUOH Chang-Sheng , CHEN Wen-Huei , CHEN Hong-Hwa
Plant and Cell Physiology 46(7), 1125-1139, 2005-07-01
References (58) Cited by (9)
-
38
- Interactions of B-class complex proteins involved in tepal development in Phalaenopsis orchid
-
TSAI Wen-Chieh , PAN Zhao-Jun , HSIAO Yu-Yun , JENG Mei-Fen , WU Ting-Feng , CHEN Wen-Huei , CHEN Hong-Hwa
Plant and Cell Physiology 49(5), 814-824, 2008-05-01
References (44) Cited by (5)
-
39
- The duplicated B class heterodimer model : whorl specific effects and complex genetic interactions in Petunia hybrida flower development
-
VANDENBUSSCHE M.
Plant Cell 16, 741-754, 2004
DOI Cited by (5)
-
40
- Conservation of B-class floral homeotic, gene function between maize and Arabidopsis
-
WHIPPLE C. J.
Development 131, 6083-6091, 2004
DOI Cited by (5)
-
41
- Conservation of B class gene expression in the second whorl of a basal grass and outgroups links the origin of lodicules and petals
-
WHIPPLE CJ
Proc Natl Acad Sci USA 104, 1081-1086, 2007
Cited by (1)
-
42
- Floral organ identity genes in the orchid Dendrobium crumenatum
-
XU Y.
Plant J. 46, 54-68, 2006
DOI Cited by (8)
-
43
- Unequal Genetic Redundancy of Rice PISTILLATA Orthologs, OsMADS2 and OsMADS4, in Lodicule and Stamen Development
-
YAO Shan-Guo , OHMORI Shinnosuke , KIMIZU Mayumi , YOSHIDA Hitoshi
Plant and Cell Physiology 49(5), 853-857, 2008-05-01
References (19) Cited by (1)