Ectopic expression of an AP3-like and a PI-like genes from 'Sekkoku' orchid (Dendrobium moniliforme) causes the homeotic conversion of sepals to petals in whorl 1 and the suppression of carpel development in whorl 4 in Arabidopsis flowers

Access this Article

Search this Article

Author(s)

Abstract

Three class-B MADS-box genes, the paleotype <i>AP3</i> genes <i>DMAP3A</i> and <i>DMAP3B,</i> and the <i>PI</i>-like gene <i>DMPI</i>, were isolated from a native orchid in Japan named ‘Sekkoku’ (<i>Dendrobium moniliforme</i>) and ectopically expressed in <i>Arabidopsis</i>. <i>DMAP3A</i> and <i>DMPI</i> were expressed in all four floral whorls in the orchid, whereas <i>DMAP3B</i> was expressed only in whorls 2, 3, and 4. The ectopic expression of 35S::<i>DMPI</i> caused a partial sepal-to-petal conversion in whorl 1, increased the longevity of the flowers and delayed silique maturation in the transgenic plants, compared with wild-type plants. Transgenic <i>Arabidopsis</i> plants over-expressing 35S::<i>DMAP3B/</i>35S::<i>DMPI</i> exhibited a vegetative phenotype with leaf curling, a near-complete sepal-to-petal conversion in whorl 1, and the suppression of carpel development in whorl 4. Our results indicate that <i>DMAP3B</i> and <i>DMPI</i> are major class B MADS-box genes in <i>D. moniliforme</i> and may play an important role in the development of petals in the second floral whorl of the orchid. In addition, our results also suggest that these genes can be used for the genetic engineering of flower shape in plants.

Journal

  • Plant Biotechnology

    Plant Biotechnology 27(2), 183-192, 2010-06-25

    Japanese Society for Plant Cell and Molecular Biology

References:  43

Codes

  • NII Article ID (NAID)
    10026527239
  • NII NACSIS-CAT ID (NCID)
    AA11250821
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    13424580
  • NDL Article ID
    10735951
  • NDL Source Classification
    ZR3(科学技術--生物学--植物)
  • NDL Call No.
    Z54-J126
  • Data Source
    CJP  NDL  J-STAGE 
Page Top