Cotyledonary node pre-wounding with a micro-brush increased frequency of Agrobacterium-mediated transformation in soybean

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Abstract

A Japanese soybean variety, Kariyutaka, known to form many adventitious shoots from sections of the cotyledonary node, had a transformation frequency (relative number of T<sub>0</sub> transgenic plants, which stably transmitted transgenes to their T<sub>1</sub> progenies, to number of explants subjected to <i>Agrobacterium</i>-infection) of about 1.0% in the conventional protocol. We modified this method to obtain the transformation frequency of 4.4%. Modified protocol included mechanical wounding of explants with a micro-brush and application of 0.02% of Silwet L-77 during the infection with <i>Agrobacterium</i>. We obtained T<sub>0</sub> plants 8 to 12 weeks after co-cultivation with <i>Agrobacterium</i>. T<sub>1</sub> seeds were rapidly obtained from 5 to 6 months after the co-cultivation, for T<sub>0</sub> plants flowered precociously. Three T<sub>0</sub> plants out of 4, i.e. 75% of all T<sub>0</sub> plants, which were generated at 12 weeks after co-cultivation, possessed stable transgene(s) that were transmitted to their T<sub>1</sub> progenies, although only 7 T<sub>0</sub> plants out of 22, i.e. 32% of all T<sub>0</sub> plants, which were generated at 8 weeks after the co-cultivation, stably transmitted the Basta-resistance to their T<sub>1</sub> progenies.

Journal

  • Plant Biotechnology

    Plant Biotechnology 27(2), 217-220, 2010-06-25

    Japanese Society for Plant Cell and Molecular Biology

References:  15

Cited by:  2

Codes

  • NII Article ID (NAID)
    10026527390
  • NII NACSIS-CAT ID (NCID)
    AA11250821
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13424580
  • NDL Article ID
    10736095
  • NDL Source Classification
    ZR3(科学技術--生物学--植物)
  • NDL Call No.
    Z54-J126
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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