ヒラメ白血球包囲化反応における顆粒球およびケモカインの活性 Activity of Granulocytes and Chemokines in the Leukocyte-encapsulation Response of Japanese Flounder Paralichthys olivaceus

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Author(s)

Abstract

Ichthyophonus hoferiの多核球状体に対するヒラメ白血球のin vitro包囲化反応では、ペルオキシダーゼ陽性の顆粒球が多数を占めていた。顆粒球はI. hoferiに接着し、その周囲を取り囲んでいた。包囲化の過程において、3種類のCCケモカインおよび1種類のCXCケモカインのmRNA発現上昇が確認された。特に、CC-CLMおよびIL-8は顆粒球により産生され、異物包囲化の初期反応における顆粒球の重要性が示唆された。

The role of leukocytes from the kidney of Japanese flounder <i>Paralichthys olivaceus</i> in the encapsulation response was investigated using an <i>in vitro</i> model. The aggregation response of leukocytes to the target fish pathogen <i>Ichthyophonus hoferi</i> was observed over 12 h. The cellular aggregates mainly comprised granulocytes, which were characterized by intracellular peroxidase expression. Moreover, studies of isolated granulocytes demonstrated their adhesion to <i>I. hoferi</i> and formation of an initial layer in the encapsulation response. We observed that mRNA expression levels of three CC-chemokines (CCL3, CCL4 and CC-CLM) and one CXC-chemokine (IL-8) increased during the encapsulation process. Among these chemokines, CC-CLM and IL-8 were actively produced by granulocytes upon stimulation by <i>I. hoferi</i>. Flounder granulocytes therefore appeared to play an important role in the process by releasing specific chemokines upon pathogen recognition, thereby inducing subsequent cellular recruitment leading to encapsulation.

Journal

  • Fish Pathology

    Fish Pathology 45(3), 121-129, 2010-09-15

    The Japanese Society of Fish Pathology

References:  51

Cited by:  1

Codes

  • NII Article ID (NAID)
    10026600679
  • NII NACSIS-CAT ID (NCID)
    AN00063165
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    0388788X
  • NDL Article ID
    10833946
  • NDL Source Classification
    ZR4(科学技術--生物学--動物)
  • NDL Call No.
    Z18-994
  • Data Source
    CJP  CJPref  NDL  J-STAGE  JASI 
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