Molecular Characterization of Fungal Communities in Non-Tilled, Cover-Cropped Upland Rice Field Soils

• Nishizawa Tomoyasu

  Ibaraki University College of Agriculture

• Zhaorigetu

  Ibaraki University College of Agriculture

• Komatsuzaki Masakazu

  Ibaraki University College of Agriculture Institute for Global Change Adaptation Science, Ibaraki University

• Sato Yoshinori

  Institute for Global Change Adaptation Science, Ibaraki University

• Kaneko Nobuhiro

  Graduate School of Environment and Information Science, Yokohama National University

• Ohta Hiroyuki

  Ibaraki University College of Agriculture Institute for Global Change Adaptation Science, Ibaraki University

Abstract

This study aimed to characterize soil fungal communities in upland rice fields managed with tillage/non-tillage and winter cover-cropping (hairy vetch and cereal rye) practices, using PCR-based molecular methods. The study plots were maintained as upland fields for 5 years and the soils sampled in the second and fifth years were analyzed using T-RFLP (terminal restriction fragment length polymorphism) profiling and clone libraries with the internal transcribed spacer (ITS) region and domain 1 (D1) of the fungal large-subunit (fLSU) rRNA (D1_fLSU) as the target DNA sequence. From the 2nd-year-sample, 372 cloned sequences of fungal ITS-D1_fLSU were obtained and clustered into 80 nonredundant fungal OTUs (operational taxonomic units) in 4 fungal phyla. The T-RFLP profiling was performed with the 2nd- and 5th-year-samples and the major T-RFs (terminal restriction fragments) were identified using a theoretical fragment analysis of the ITS-D1_fLSU clones. These molecular analyses showed that the fungal community was influenced more strongly by the cover-cropping than tillage practices. Moreover, the non-tilled, cover-cropped soil was characterized by a predominance of Cryptococcus sp. in the phylum Basidiomycota. We provided a genetic database of the fungal ITS-D1_fLSUs in the differently managed soils of upland rice fields.<br>

Journal

• Microbes and Environments

  Microbes and Environments 25 (3), 204-210, 2010

  Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

Keywords

• clone library
• <i>Cryptococcus</i>
• soil fungal community
• T-RFLP
• ribosomal RNA gene

Details

• CRID: 1390001204344779776
• NII Article ID: 10026643423
• NII Book ID: AA11551577
• DOI: 10.1264/jsme2.me10108
• ISSN: 13474405
• ISSN: 13426311
• NDL BIB ID: 10810496
• Web Site: http://id.ndl.go.jp/bib/10810496
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10810496
• Web Site: http://www.jstage.jst.go.jp/article/jsme2/25/3/25_ME10108/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed