Real-time PCR detection of host-mediated cyanophage gene transcripts during infection of a natural <italic>Microcystis aeruginosa</italic> population

• Yoshida Mitsuhiro

  Division of the Aquatic Biology and Ecology, Center for Marine Environmental Studies (CMES), Ehime University

• Yoshida Takashi

  Department of Agriculture, Kyoto University

• Yoshida-Takashima Yukari

  Subsurface Geobiology Advanced Research (SUGAR) Team, Extremobiosphere Research Program, Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)

• Kashima Aki

  Department of Marine Bioscience, Fukui Prefectural University

• Hiroishi Shingo

  Department of Marine Bioscience, Fukui Prefectural University

書誌事項

タイトル別名

• Real-Time PCR Detection of Host-Mediated Cyanophage Gene Transcripts during Infection of a Natural Microcystis aeruginosa Population

抄録

The aim of this study was to develop a quantitative real-time reverse transcription-PCR (real-time RT-PCR) assay to detect and quantify mRNA of cyanophages within infected Microcystis aeruginosa cells in a freshwater pond. Laboratory-based data showed that the relative abundance of the cyanophage g91 mRNA within host cells increased before cyanophage numbers increased in culture. This transcriptional pattern indicated the kinetics of the viral infection suggesting the real-time RT-PCR method to be a potential tool for environmental monitoring of cyanophage infections. In this field survey, the numbers of infected M. aeruginosa cell populations estimated from cyanophage numbers were low at 0.01–2.9 cells mL⁻¹. The highest relative abundance of phage g91 RNA (10⁻² per rnpB transcript) was at about the same levels of expression as laboratory-based growth data for Ma-LMM01 (estimated density of infected host cells: 10⁵ cells mL⁻¹); and was observed when cyanophage numbers rapidly increased (as well as a decrease in host cell numbers). Quantification of cyanophage numbers is important to understand ecological relationships between the phage and its hosts. Our data suggest the quantification of phage gene transcripts within a natural host cell population to be a strong tool for investigating the quantitative effects of phage lysis during infection of the host population.<br>

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 25 (3), 211-215, 2010

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• cyanophage
• <i>Microcystis aeruginosa</i>
• real-time RT-PCR
• succession
• toxic cyanobacteria

詳細情報

• CRID: 1390001204344783232
• NII論文ID: 10026643464
• NII書誌ID: AA11173196
• DOI: 10.1264/jsme2.me10117
• ISSN: 13474405
• ISSN: 13426311
• HANDLE: 2433/130694
• NDL書誌ID: 10810516
• Web Site: http://id.ndl.go.jp/bib/10810516
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10810516
• Web Site: http://www.jstage.jst.go.jp/article/jsme2/25/3/25_ME10117/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可