An <i>in Vitro</i> Evaluation Method to Test Ocular Irritation using a Human Corneal Epithelium Model
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- Arai Shoko
- Japanese Center for the Validation of Alternative Methods (JaCVAM), National Institute of Health Sciences
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- Yamamoto Naoki
- Fujita Health University
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- Katoh Masakazu
- Japan Tissue Engineering Co., Ltd.
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- Kojima Hajime
- Japanese Center for the Validation of Alternative Methods (JaCVAM), National Institute of Health Sciences
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抄録
Recently, safety evaluation tests that do not involve animal experiments have been prosperously developing. However, the optimal evaluation materials and methods for assessing ocular irritancy have not been well investigated. In this study, we determined the optimal evaluation method for testing ocular irritation using a human cultured corneal epithelium model (corneal model). In order to assess adequate treatment conditions for the corneal model, we used cetylpyridinium chloride (CPC), which has been recognized as an irritant chemical by the Draize eye test. The irritancy elicited by multiple concentrations of CPC was evaluated by a cytotoxicity assay under nine treatment conditions and compared to the Draize score. The treatment conditions that included a 5-second exposure period followed by a 24-hour post-incubation period (hereafter called protocol "5-sec+24-h") showed a significant correlation between cytotoxicity and the Draize score. Furthermore, the dose-dependent cytotoxicity of six test chemicals was assessed by protocol "5-sec+24-h" and found to correlate with the Draize eye test results.
収録刊行物
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- Alternatives to Animal Testing and Experimentation
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Alternatives to Animal Testing and Experimentation 13 (2), 83-90, 2008
日本動物実験代替法学会
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詳細情報 詳細情報について
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- CRID
- 1390282680211499776
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- NII論文ID
- 10026777428
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- NII書誌ID
- AA11161787
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- ISSN
- 21854726
- 13440411
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可