Capillary Chromatography Based on the Tube Radial Distribution of Aqueous-Organic Mixture Carrier Solvents: Effects of the Inner-Wall Characteristics of the Fused-Silica Tube on Separation Performance

• JINNO Naoya

  Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

• HASHIMOTO Ko

  Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

• HASHIMOTO Masahiko

  Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

• TSUKAGOSHI Kazuhiko

  Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

Abstract

A capillary chromatography system was developed using an open capillary tube and a water-acetonitrile (hydrophilic)-ethyl acetate (hydrophobic) mixture carrier solution. In this study, we examined the effects of the inner-wall characteristics of a fused-silica capillary tube on the separation performance in this system. Untreated (silanol group-intact) and inactivated (silanol group-end-blocked) fused-silica capillary tubes were used. The mixture analyte of 1-naphthol and 2,6-naphthalenedisulfonic acid was injected into two types of capillary tubes. They were eluted from both tubes in this order with water-acetonitrile-ethyl acetate (volume ratio 2:7:4) mixture carrier solution, and eluted in reverse order with water-acetonitrile-ethyl acetate (volume ratio 15:3:2). The peak shapes observed here were analyzed while considering the inner-wall characteristics of the capillary tubes from the viewpoint of the tube radial distribution of the carrier solvents in the tubes.

Journal

• Analytical Sciences

  Analytical Sciences 25 (11), 1369-1371, 2009

  The Japan Society for Analytical Chemistry

Keywords

• Analytical Chemistry

Details

• CRID: 1390282679232775552
• NII Article ID: 130004441788
• NII Article ID: 10026780198
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.25.1369
• COI: 1:STN:280:DC%2BD1MjlsVygug%3D%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 10425365
• PubMed: 19907097
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10425365
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.25.1369.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.25.1369/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/25/11/25_11_1369/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed