Separation of Optical Isomers in Capillary Chromatography Using a Poly(tetrafluoroethylene) Capillary Tube and an Aqueous-Organic Mixture Carrier Solution
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- ISHIMOTO Seiji
- Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University
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- JINNO Naoya
- Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University
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- HASHIMOTO Masahiko
- Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University
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- TSUKAGOSHI Kazuhiko
- Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University
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Abstract
Capillary chromatography for the separation of optical isomers was developed using an untreated poly(tetrafluoroethylene) capillary tube and a water–hydrophilic/hydrophobic organic solvent mixture as a carrier solution. The open tubular capillary was 110 cm in length (90 cm effective length) and 100 μm in inner diameter. The carrier solution was prepared with a water–acetonitrile–ethyl acetate mixture (15:3:2 volume ratio) containing 1 mM β-cyclodextrin. A model analyte solution of dansyl-DL-methionine was injected into the capillary tube by a gravity method. The analyte solution was subsequently delivered through the capillary tube with the carrier solution by a microsyringe pump; the system worked under laminar-flow conditions. The analytes were separated through the capillary tube with on-capillary detection by an absorption detector. D-Isomer and L-isomer were eluted in this order with the water-acetonitrile–ethyl acetate carrier solution including β-cyclodextrin.
Journal
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- Analytical Sciences
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Analytical Sciences 26 (6), 641-643, 2010
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390282679233337856
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- NII Article ID
- 10026783869
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- NII Book ID
- AA10500785
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- COI
- 1:STN:280:DC%2BC3cvpvFarsA%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 10703832
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- PubMed
- 20543493
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed