Aptamer-Mediated Chemiluminescence Detection of Prion Protein on a Membrane Using Trimethoxyphenylglyoxal

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  • HOSSAIN Md Towhid
    Faculty of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University
  • SHIBATA Takayuki
    Faculty of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University
  • KABASHIMA Tsutomu
    Faculty of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University
  • KAI Masaaki
    Faculty of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University Global Center of Excellence Program, Nagasaki University

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Abstract

Effective recognition and quantitative analysis of the prion protein are important in drug discovery and diagnosis for prion diseases, such as bovine spongiform encephalopathy and Creutzfeldt-Jakob diseases. We have developed a high-throughput method for a specific and sensitive determination of prion protein on a solid-phase membrane, based on a chemiluminescence reaction of aptamer with 3,4,5-trimethoxyphenylglyoxal. This method using aptamer is facile, inexpensive and convenient for the detection of the prion protein on a membrane, indicating a lower detection limit of ca. 4.2 pmol spot−1.

Journal

  • Analytical Sciences

    Analytical Sciences 26 (6), 645-647, 2010

    The Japan Society for Analytical Chemistry

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