Analytical Conditions and Separation Performance of Capillary Chromatography Based on the Tube Radial Distribution of Aqueous-Organic Mixture Carrier Solvents under Laminar-Flow Conditions

• JINNO Naoya

  Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

• MURAKAMI Mari

  Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

• HASHIMOTO Masahiko

  Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

• TSUKAGOSHI Kazuhiko

  Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

Abstract

We have developed a capillary chromatography system using an open capillary tube made of fused-silica, polyethylene, or poly(tetrafluoroethylene), and a water–hydrophilic–hydrophobic organic mixture carrier solution. This tube radial distribution chromatography (TRDC) system works under laminar-flow conditions. In this study, the following analytical conditions in the TRDC system using a fused-silica capillary tube and a water–acetonitrile–ethyl acetate mixture carrier solution were for the first time examined: tube temperature, 5 − 25°C; tube inner diameter, 50 − 250 μm; tube effective length, 100 − 200 cm; and flow rate, 0.2 − 1.5 μL min⁻¹. For example, the effects of temperature on the separation performance in the TRDC system were observed with an organic solvent-rich carrier solution; 1-naphthol and 2,6-naphthalenedisulfonic acid in a model mixture were eluted with baseline separation over the temperature range of 5 − 23°C. The resolution, theoretical plate number, and height equivalent to the theoretical plate were calculated from the experimental data obtained by examining the effects of the tube length. A mixture of 1-naphthol, Eosin Y, 1-naphthalenesulfonic acid, 2,6-naphthalenedisulfonic acid, and 1,3,6-naphthalenetrisulfonic acid was subjected to the present TRDC system, and the analytes in the mixture solution were eluted in this order with the organic solvent-rich carrier solution, providing good separation performance on the chromatogram.

Journal

• Analytical Sciences

  Analytical Sciences 26 (7), 737-742, 2010

  The Japan Society for Analytical Chemistry

Keywords

• Analytical Chemistry

Details

• CRID: 1390001204258352512
• NII Article ID: 10026784316
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.26.737
• COI: 1:STN:280:DC%2BC3cnntlSntg%3D%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 10744383
• PubMed: 20631432
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10744383
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.26.737.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.26.737/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/26/7/26_7_737/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed