Application of an Oxygen Electrode to Evaluate Superoxide Anion-scavenging Ability
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- KOMAGOE Keiko
- Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- TAKEUCHI Hiroaki
- Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- INOUE Tsuyoshi
- Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- KATSU Takashi
- Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Abstract
The ability to scavenge superoxide anion radicals (•O2−) was determined using an oxygen electrode. The method is based on the determination of •O2− generated by the reaction of nitrilotriacetatoiron(III) with hydrogen peroxide and a decrease in the concentration of •O2− by a scavenging reaction, converting into a change in the generation of oxygen molecules through an electron-transfer reaction from •O2− to nitrilotriacetatoiron(III). Oxygen generation, which enhanced proportionally with an increase in the concentration of hydrogen peroxide, was inhibited depending on the concentration of superoxide dismutase. Hence, we applied the present reaction system to evaluate the •O2−-scavenging abilities of an antioxidant, measuring the degree of inhibition of oxygen generation using an oxygen electrode. A good correlation was obtained between the present method and conventional colorimetry, monitoring the formation of blueformazan by the reaction of nitro blue tetrazolium with •O2−, to estimate the •O2−-scavenging activities of antioxidants.
Journal
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- Analytical Sciences
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Analytical Sciences 26 (8), 903-906, 2010
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390001204260622336
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- NII Article ID
- 10026784989
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- NII Book ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 10779973
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed